Detailed information of TA system TA097727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 40648..41233
• Replicon: plasmid pOXA58_005078
• Accession: NZ_CP027245
• Organism: Acinetobacter baumannii strain WCHAB005078

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: C6N18_RS00765
• Protein ID: WP_000897307.1
• Coordinates: 40648..40968 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6N18_RS00770
• Protein ID: WP_000369781.1
• Coordinates: 40961..41233 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N18_RS00725	36006..36959	-	954	Protein_35	HAMP domain-containing protein	-
C6N18_RS00730	36949..37677	-	729	WP_032073130.1	efflux system response regulator transcription factor AdeR	-
C6N18_RS00735	38069..38356	+	288	WP_000438826.1	BrnT family toxin	-
C6N18_RS00740	38343..38657	+	315	WP_001983304.1	BrnA antitoxin family protein	-
C6N18_RS00745	38746..39276	-	531	WP_000172759.1	hypothetical protein	-
C6N18_RS00750	39425..39727	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C6N18_RS00755	39728..40009	+	282	WP_000985609.1	putative addiction module antidote protein	-
C6N18_RS00760	40089..40508	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
C6N18_RS00765	40648..40968	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6N18_RS00770	40961..41233	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
C6N18_RS00775	41726..43201	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
C6N18_RS00780	43257..44141	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
C6N18_RS00785	44332..44580	-	249	WP_087548421.1	helix-turn-helix domain-containing protein	-
C6N18_RS00795	44600..45304	+	705	WP_005028420.1	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / blaOXA-58 / msr(E) / mph(E) / floR	-	1..70509	70509
	-	inside	IScluster/Tn	msr(E) / mph(E) / floR	-	41726..53345	11619

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T97727 WP_000897307.1 NZ_CP027245:40648-40968 [Acinetobacter baumannii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T97727 NZ_CP027245:40648-40968 [Acinetobacter baumannii]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT97727 WP_000369781.1 NZ_CP027245:40961-41233 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT97727 NZ_CP027245:40961-41233 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3