Detailed information of TA system TA097700

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 14323..14749
• Replicon: plasmid unnamed1
• Accession: NZ_CP027222
• Organism: Escherichia coli strain 2015C-3101

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C6H98_RS28360
• Protein ID: WP_001312861.1
• Coordinates: 14323..14481 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	14525..14749 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6H98_RS28320	9693..10382	-	690	WP_000283394.1	conjugal transfer transcriptional regulator TraJ	-
C6H98_RS28325	10569..10952	-	384	WP_001151524.1	relaxosome protein TraM	-
C6H98_RS28330	11366..11875	+	510	Protein_14	transglycosylase SLT domain-containing protein	-
C6H98_RS28335	12172..12993	-	822	WP_001234469.1	DUF945 domain-containing protein	-
C6H98_RS28340	13113..13401	-	289	Protein_16	hypothetical protein	-
C6H98_RS29715	13745..13969	+	225	WP_001427866.1	hypothetical protein	-
C6H98_RS28360	14323..14481	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	14525..14749	-	225	NuclAT_0	-	Antitoxin
-	14525..14749	-	225	NuclAT_0	-	Antitoxin
-	14525..14749	-	225	NuclAT_0	-	Antitoxin
-	14525..14749	-	225	NuclAT_0	-	Antitoxin
C6H98_RS29450	14561..14749	+	189	WP_001299721.1	hypothetical protein	-
C6H98_RS28365	14761..15480	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
C6H98_RS28370	15477..15911	-	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
C6H98_RS28375	15966..17924	-	1959	WP_001145472.1	ParB/RepB/Spo0J family partition protein	-
C6H98_RS28380	17983..18216	-	234	WP_000006004.1	DUF905 domain-containing protein	-
C6H98_RS28385	18272..18799	-	528	WP_000290792.1	single-stranded DNA-binding protein	-
C6H98_RS28400	19144..19368	+	225	WP_001427866.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / hlyA / hlyB / hlyD / hlyD	1..48390	48390

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T97700 WP_001312861.1 NZ_CP027222:c14481-14323 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T97700 NZ_CP027222:c14481-14323 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT97700 NZ_CP027222:c14749-14525 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin