Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/PHD-PIN |
| Location | 3262064..3262749 | Replicon | chromosome |
| Accession | NC_000911 | ||
| Organism | Synechocystis sp. PCC 6803 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | A0A6P1VMJ8 |
| Locus tag | sll0525 | Protein ID | WP_010874101.1 |
| Coordinates | 3262064..3262459 (-) | Length | 132 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | - |
| Locus tag | ssl1004 | Protein ID | WP_010874102.1 |
| Coordinates | 3262471..3262749 (-) | Length | 93 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SGL_RS16950 (sll0529) | 3257692..3259887 | - | 2196 | WP_010874098.1 | phosphoketolase | - |
| SGL_RS16955 (sll0528) | 3260137..3261276 | - | 1140 | WP_010874099.1 | site-2 protease family protein | - |
| SGL_RS16960 (slr0556) | 3261406..3262062 | + | 657 | WP_010874100.1 | YggS family pyridoxal phosphate-dependent enzyme | - |
| SGL_RS16965 (sll0525) | 3262064..3262459 | - | 396 | WP_010874101.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| SGL_RS16970 (ssl1004) | 3262471..3262749 | - | 279 | WP_010874102.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| SGL_RS16975 (sll0524) | 3262819..3264162 | - | 1344 | WP_010874103.1 | FIST C-terminal domain-containing protein | - |
| SGL_RS16980 (slr0557) | 3264290..3267022 | + | 2733 | WP_010874104.1 | valine--tRNA ligase | - |
| SGL_RS16985 (sll0522) | 3267244..3267549 | - | 306 | WP_197525966.1 | NADH-quinone oxidoreductase subunit NuoK | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 132 a.a. Molecular weight: 15081.50 Da Isoelectric Point: 4.5594
>T977 WP_010874101.1 NC_000911:c3262459-3262064 [Synechocystis sp. PCC 6803]
VKLLLDTQCWLWWFTEPEKLNEDAISQIIDETNEIWLSVASIWEMSIKTSIGKLPLPENIDDYILSRMVKLGAEFLDIKT
RHALQVATLPLHHRDPFDRIIIAQAQTENMLLISADSMFKNYGVSLLWAGV
VKLLLDTQCWLWWFTEPEKLNEDAISQIIDETNEIWLSVASIWEMSIKTSIGKLPLPENIDDYILSRMVKLGAEFLDIKT
RHALQVATLPLHHRDPFDRIIIAQAQTENMLLISADSMFKNYGVSLLWAGV
Download Length: 396 bp
>T977 NC_000911:c3262459-3262064 [Synechocystis sp. PCC 6803]
GTGAAACTTTTGTTGGATACCCAGTGCTGGTTATGGTGGTTTACTGAGCCGGAAAAATTGAACGAAGATGCAATTTCCCA
GATCATAGATGAGACAAACGAAATATGGCTTTCTGTTGCCAGTATTTGGGAAATGTCAATTAAGACTTCCATCGGTAAAC
TGCCCTTGCCAGAAAATATTGACGACTACATTCTCAGTCGCATGGTGAAATTGGGTGCTGAGTTCCTGGACATTAAAACC
CGCCATGCCCTGCAAGTTGCGACATTGCCCCTACATCACCGTGATCCGTTTGACAGAATTATCATTGCCCAGGCACAAAC
AGAAAATATGCTGCTAATTAGTGCAGATTCAATGTTTAAAAATTATGGAGTCTCTTTGTTATGGGCGGGAGTATAG
GTGAAACTTTTGTTGGATACCCAGTGCTGGTTATGGTGGTTTACTGAGCCGGAAAAATTGAACGAAGATGCAATTTCCCA
GATCATAGATGAGACAAACGAAATATGGCTTTCTGTTGCCAGTATTTGGGAAATGTCAATTAAGACTTCCATCGGTAAAC
TGCCCTTGCCAGAAAATATTGACGACTACATTCTCAGTCGCATGGTGAAATTGGGTGCTGAGTTCCTGGACATTAAAACC
CGCCATGCCCTGCAAGTTGCGACATTGCCCCTACATCACCGTGATCCGTTTGACAGAATTATCATTGCCCAGGCACAAAC
AGAAAATATGCTGCTAATTAGTGCAGATTCAATGTTTAAAAATTATGGAGTCTCTTTGTTATGGGCGGGAGTATAG
Antitoxin
Download Length: 93 a.a. Molecular weight: 10084.59 Da Isoelectric Point: 4.0201
>AT977 WP_010874102.1 NC_000911:c3262749-3262471 [Synechocystis sp. PCC 6803]
MIGVSVNPEKLGSTMETVNVDQIEINLPELLYSIKPGEEVIVADQGIPIAKLVPLQRQKSVDRCSSLGVDRGLFVVPDDF
NDPLPNDIWPLS
MIGVSVNPEKLGSTMETVNVDQIEINLPELLYSIKPGEEVIVADQGIPIAKLVPLQRQKSVDRCSSLGVDRGLFVVPDDF
NDPLPNDIWPLS
Download Length: 279 bp
>AT977 NC_000911:c3262749-3262471 [Synechocystis sp. PCC 6803]
ATGATCGGAGTTAGCGTTAATCCAGAGAAACTAGGATCCACTATGGAGACCGTCAATGTTGACCAAATAGAAATCAATCT
TCCTGAGCTTTTGTACAGTATCAAACCAGGGGAAGAAGTCATCGTTGCTGACCAGGGTATTCCCATTGCTAAGTTGGTTC
CTCTGCAAAGGCAAAAATCCGTGGATCGATGCTCCAGCTTAGGGGTAGATCGAGGATTATTTGTAGTGCCAGATGACTTT
AACGATCCTTTACCAAATGATATTTGGCCATTATCTTGA
ATGATCGGAGTTAGCGTTAATCCAGAGAAACTAGGATCCACTATGGAGACCGTCAATGTTGACCAAATAGAAATCAATCT
TCCTGAGCTTTTGTACAGTATCAAACCAGGGGAAGAAGTCATCGTTGCTGACCAGGGTATTCCCATTGCTAAGTTGGTTC
CTCTGCAAAGGCAAAAATCCGTGGATCGATGCTCCAGCTTAGGGGTAGATCGAGGATTATTTGTAGTGCCAGATGACTTT
AACGATCCTTTACCAAATGATATTTGGCCATTATCTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T972 | Synechocystis sp. PCC 6803 |
30.952 |
98.438 |
0.305 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Stefan Kopfmann et al. (2016) Type II Toxin-Antitoxin Systems in the Unicellular Cyanobacterium Synechocystis sp. PCC 6803. Toxins 8(7):228. [PubMed:27455323]