Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/Xre-PIN |
| Location | 1933468..1934060 | Replicon | chromosome |
| Accession | NC_000911 | ||
| Organism | Synechocystis sp. PCC 6803 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | - |
| Locus tag | SGL_RS11010 | Protein ID | WP_010872957.1 |
| Coordinates | 1933468..1933854 (-) | Length | 129 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | - |
| Locus tag | SGL_RS11015 | Protein ID | WP_010872958.1 |
| Coordinates | 1933854..1934060 (-) | Length | 69 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SGL_RS11000 (slr1165) | 1931139..1932311 | + | 1173 | WP_010872955.1 | sulfate adenylyltransferase | - |
| SGL_RS11005 (slr1166) | 1932354..1933418 | + | 1065 | WP_010872956.1 | glycosyltransferase | - |
| SGL_RS11010 (sll1092) | 1933468..1933854 | - | 387 | WP_010872957.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| SGL_RS11015 (ssl2138) | 1933854..1934060 | - | 207 | WP_010872958.1 | DUF2281 domain-containing protein | Antitoxin |
| SGL_RS11020 (sll1091) | 1934307..1935530 | - | 1224 | WP_010872959.1 | geranylgeranyl reductase | - |
| SGL_RS11025 (slr1167) | 1935912..1937069 | + | 1158 | WP_010872960.1 | oxidoreductase | - |
| SGL_RS11030 (slr1168) | 1937086..1937397 | + | 312 | WP_010872961.1 | DUF433 domain-containing protein | - |
| SGL_RS11035 (slr1169) | 1937474..1938310 | + | 837 | WP_010872962.1 | DUF1049 domain-containing protein | - |
| SGL_RS11040 (slr1170) | 1938429..1938782 | + | 354 | WP_010872963.1 | TMEM165/GDT1 family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 129 a.a. Molecular weight: 14814.14 Da Isoelectric Point: 6.0713
>T972 WP_010872957.1 NC_000911:c1933854-1933468 [Synechocystis sp. PCC 6803]
MNSVLIDTHVFIWLAEDDSNLPVTTKDTLENMDNVFVSIASFWEISIKLKIGKLSLCGDFNSIEASFQRTRFKLLPITLK
DTIKLYNLPLHHKDPFDRILVSQSINHSLILVSRDQALDDYPIQRLWL
MNSVLIDTHVFIWLAEDDSNLPVTTKDTLENMDNVFVSIASFWEISIKLKIGKLSLCGDFNSIEASFQRTRFKLLPITLK
DTIKLYNLPLHHKDPFDRILVSQSINHSLILVSRDQALDDYPIQRLWL
Download Length: 387 bp
>T972 NC_000911:c1933854-1933468 [Synechocystis sp. PCC 6803]
ATGAATTCTGTTTTGATAGACACCCACGTTTTTATTTGGTTAGCAGAAGATGACTCTAACTTGCCTGTGACGACAAAAGA
TACCTTAGAAAATATGGATAACGTTTTTGTTAGTATTGCCAGCTTTTGGGAAATTTCTATTAAACTAAAAATTGGTAAAC
TTTCTCTCTGTGGTGATTTCAATAGTATTGAAGCTAGTTTTCAAAGAACCCGATTCAAGCTATTACCTATTACACTAAAA
GACACAATTAAACTTTATAATCTTCCCTTGCACCACAAAGATCCCTTTGATCGTATTTTGGTATCCCAATCGATTAATCA
TTCTCTTATTTTAGTCAGTCGTGATCAAGCTCTGGATGATTATCCGATTCAACGCCTGTGGTTGTAG
ATGAATTCTGTTTTGATAGACACCCACGTTTTTATTTGGTTAGCAGAAGATGACTCTAACTTGCCTGTGACGACAAAAGA
TACCTTAGAAAATATGGATAACGTTTTTGTTAGTATTGCCAGCTTTTGGGAAATTTCTATTAAACTAAAAATTGGTAAAC
TTTCTCTCTGTGGTGATTTCAATAGTATTGAAGCTAGTTTTCAAAGAACCCGATTCAAGCTATTACCTATTACACTAAAA
GACACAATTAAACTTTATAATCTTCCCTTGCACCACAAAGATCCCTTTGATCGTATTTTGGTATCCCAATCGATTAATCA
TTCTCTTATTTTAGTCAGTCGTGATCAAGCTCTGGATGATTATCCGATTCAACGCCTGTGGTTGTAG
Antitoxin
Download Length: 69 a.a. Molecular weight: 7699.84 Da Isoelectric Point: 3.8804
>AT972 WP_010872958.1 NC_000911:c1934060-1933854 [Synechocystis sp. PCC 6803]
MTTEAALLNIAENLPDDLKIELLHYAEYLMNKSLNAPQKPLAGSMKGTFILPLPEDFDAPLEEFEEYM
MTTEAALLNIAENLPDDLKIELLHYAEYLMNKSLNAPQKPLAGSMKGTFILPLPEDFDAPLEEFEEYM
Download Length: 207 bp
>AT972 NC_000911:c1934060-1933854 [Synechocystis sp. PCC 6803]
ATGACTACTGAAGCTGCCCTATTAAACATTGCTGAAAATCTGCCTGATGACTTAAAAATCGAGCTTTTACACTATGCTGA
ATATTTAATGAATAAATCCTTAAATGCGCCCCAAAAGCCATTAGCAGGATCGATGAAAGGAACTTTTATTTTACCTCTAC
CCGAAGACTTTGATGCCCCCTTAGAAGAATTTGAGGAGTATATGTAA
ATGACTACTGAAGCTGCCCTATTAAACATTGCTGAAAATCTGCCTGATGACTTAAAAATCGAGCTTTTACACTATGCTGA
ATATTTAATGAATAAATCCTTAAATGCGCCCCAAAAGCCATTAGCAGGATCGATGAAAGGAACTTTTATTTTACCTCTAC
CCGAAGACTTTGATGCCCCCTTAGAAGAATTTGAGGAGTATATGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T977 | Synechocystis sp. PCC 6803 |
30.952 |
98.438 |
0.305 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Degang Ning et al. (2013) [A hypothetical gene pair, ssl2138 and sll11092, constitutes a functional TA system on the chromosome of Synechocystis PCC 6803]. Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica 53(10):1043-9. [PubMed:24409759]
(2) Stefan Kopfmann et al. (2016) Type II Toxin-Antitoxin Systems in the Unicellular Cyanobacterium Synechocystis sp. PCC 6803. Toxins 8(7):228. [PubMed:27455323]