Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3157386..3157888 Replicon chromosome
Accession NZ_CP027221
Organism Escherichia coli strain 2015C-3101

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C6H98_RS17055 Protein ID WP_000767829.1
Coordinates 3157386..3157640 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C6H98_RS17060 Protein ID WP_001259255.1
Coordinates 3157637..3157888 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6H98_RS17025 3152401..3152628 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
C6H98_RS17030 3152642..3153700 - 1059 WP_000492340.1 transport protein YeeE -
C6H98_RS17035 3153879..3155237 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C6H98_RS17040 3155227..3155289 - 63 WP_010723108.1 membrane protein YoeI -
C6H98_RS17045 3155504..3156433 - 930 WP_000803331.1 LysR family transcriptional regulator -
C6H98_RS17050 3156479..3157303 - 825 WP_000754755.1 SDR family oxidoreductase -
C6H98_RS17055 3157386..3157640 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C6H98_RS17060 3157637..3157888 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C6H98_RS17065 3158172..3158222 + 51 WP_001364200.1 his operon leader peptide -
C6H98_RS17075 3158368..3159267 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C6H98_RS17080 3159273..3160577 + 1305 WP_001345258.1 histidinol dehydrogenase -
C6H98_RS17085 3160574..3161653 + 1080 WP_000102292.1 histidinol-phosphate transaminase -
C6H98_RS17090 3161650..3162717 + 1068 WP_000080054.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T97694 WP_000767829.1 NZ_CP027221:c3157640-3157386 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T97694 NZ_CP027221:c3157640-3157386 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT97694 WP_001259255.1 NZ_CP027221:c3157888-3157637 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT97694 NZ_CP027221:c3157888-3157637 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References