Detailed information of TA system TA097680

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2302584..2302955
• Replicon: chromosome
• Accession: NZ_CP027221
• Organism: Escherichia coli strain 2015C-3101

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: C6H98_RS12180
• Protein ID: WP_001443846.1
• Coordinates: 2302806..2302955 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2302584..2302762 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6H98_RS12155	2298539..2299912	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
C6H98_RS12160	2300041..2300976	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
C6H98_RS12165	2301028..2302263	-	1236	WP_000040839.1	site-specific integrase	-
C6H98_RS12170	2302265..2302480	-	216	WP_000079604.1	excisionase XisR	-
C6H98_RS12175	2302580..2302768	-	189	WP_001302840.1	DUF1187 family protein	-
-	2302584..2302762	+	179	NuclAT_0	-	Antitoxin
-	2302584..2302762	+	179	NuclAT_0	-	Antitoxin
-	2302584..2302762	+	179	NuclAT_0	-	Antitoxin
-	2302584..2302762	+	179	NuclAT_0	-	Antitoxin
C6H98_RS12180	2302806..2302955	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
C6H98_RS12185	2303011..2303820	-	810	WP_000166313.1	recombination protein RecT	-
C6H98_RS12190	2303813..2306413	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
C6H98_RS12195	2306515..2306790	-	276	WP_001344816.1	hypothetical protein	-
C6H98_RS12200	2306865..2307035	-	171	WP_001352098.1	conserved protein, Rac prophage	-
C6H98_RS12205	2307035..2307256	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleH2 / nleF / espM1 / paa / nleG7'	2301028..2390864	89836

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T97680 WP_001443846.1 NZ_CP027221:c2302955-2302806 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T97680 NZ_CP027221:c2302955-2302806 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT97680 NZ_CP027221:2302584-2302762 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin