Detailed information of TA system TA097671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 15776..16378
• Replicon: chromosome
• Accession: NZ_CP027221
• Organism: Escherichia coli strain 2015C-3101

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6H98_RS00080
• Protein ID: WP_000897305.1
• Coordinates: 15776..16087 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6H98_RS00085
• Protein ID: WP_000356397.1
• Coordinates: 16088..16378 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6H98_RS00050	10806..11591	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
C6H98_RS00055	11690..12289	+	600	WP_001315111.1	glucose-1-phosphatase	-
C6H98_RS00060	12283..13155	+	873	WP_000920762.1	virulence factor BrkB family protein	-
C6H98_RS00065	13152..13589	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6H98_RS00070	13634..14575	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6H98_RS00075	14639..15547	-	909	WP_001162704.1	alpha/beta hydrolase	-
C6H98_RS00080	15776..16087	+	312	WP_000897305.1	hypothetical protein	Toxin
C6H98_RS00085	16088..16378	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6H98_RS00095	16983..17201	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C6H98_RS00100	17420..17662	+	243	WP_001086388.1	hypothetical protein	-
C6H98_RS00105	17993..18922	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C6H98_RS00110	18919..19554	-	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
C6H98_RS00115	19551..20453	-	903	WP_000331369.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T97671 WP_000897305.1 NZ_CP027221:15776-16087 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T97671 NZ_CP027221:15776-16087 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT97671 WP_000356397.1 NZ_CP027221:16088-16378 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT97671 NZ_CP027221:16088-16378 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7