97667

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4717351..4717848 Replicon chromosome
Accession NZ_CP027219
Organism Escherichia coli strain 2015C-3163

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag C6H73_RS25735 Protein ID WP_001173343.1
Coordinates 4717351..4717524 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag C6H73_RS25740 Protein ID WP_000398619.1
Coordinates 4717552..4717848 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6H73_RS25725 4714021..4715478 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
C6H73_RS25730 4715715..4717232 + 1518 WP_001295074.1 lysine--tRNA ligase -
C6H73_RS25735 4717351..4717524 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
C6H73_RS25740 4717552..4717848 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
C6H73_RS25750 4718075..4718347 - 273 WP_000405647.1 N-acetyltransferase -
C6H73_RS25755 4718359..4718589 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
C6H73_RS25760 4718770..4720401 + 1632 WP_001216487.1 sensor histidine kinase -
C6H73_RS25765 4720398..4721117 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4708497..4717848 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T97667 WP_001173343.1 NZ_CP027219:c4717524-4717351 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T97667 NZ_CP027219:c4717524-4717351 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT97667 WP_000398619.1 NZ_CP027219:c4717848-4717552 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT97667 NZ_CP027219:c4717848-4717552 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References