Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4092502..4093043 Replicon chromosome
Accession NZ_CP027219
Organism Escherichia coli strain 2015C-3163

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag C6H73_RS22595 Protein ID WP_000615976.1
Coordinates 4092765..4093043 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C6H73_RS22590 Protein ID WP_000729704.1
Coordinates 4092502..4092762 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6H73_RS22565 4088285..4089070 - 786 WP_000207567.1 putative lateral flagellar export/assembly protein LafU -
C6H73_RS22570 4089042..4090754 + 1713 Protein_4103 flagellar biosynthesis protein FlhA -
C6H73_RS22575 4090859..4091137 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
C6H73_RS22580 4091130..4091486 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
C6H73_RS22585 4091543..4092316 - 774 WP_000543899.1 C40 family peptidase -
C6H73_RS22590 4092502..4092762 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C6H73_RS22595 4092765..4093043 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C6H73_RS22600 4093199..4093939 + 741 WP_001225679.1 murein L,D-transpeptidase -
C6H73_RS22605 4093910..4094677 - 768 WP_000333380.1 class II glutamine amidotransferase -
C6H73_RS22610 4094883..4095461 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T97663 WP_000615976.1 NZ_CP027219:4092765-4093043 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T97663 NZ_CP027219:4092765-4093043 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT97663 WP_000729704.1 NZ_CP027219:4092502-4092762 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT97663 NZ_CP027219:4092502-4092762 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References