97648

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1706771..1707273 Replicon chromosome
Accession NZ_CP027219
Organism Escherichia coli strain 2015C-3163

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C6H73_RS08945 Protein ID WP_000767829.1
Coordinates 1707019..1707273 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C6H73_RS08940 Protein ID WP_001259255.1
Coordinates 1706771..1707022 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6H73_RS08910 1701949..1703016 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C6H73_RS08915 1703016..1704086 - 1071 WP_000108980.1 histidinol-phosphate transaminase -
C6H73_RS08920 1704083..1705387 - 1305 WP_001298841.1 histidinol dehydrogenase -
C6H73_RS08925 1705393..1706292 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C6H73_RS08935 1706438..1706488 - 51 WP_001368802.1 his operon leader peptide -
C6H73_RS08940 1706771..1707022 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C6H73_RS08945 1707019..1707273 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C6H73_RS08950 1707356..1708180 + 825 WP_000754737.1 SDR family oxidoreductase -
C6H73_RS08955 1708226..1709155 + 930 WP_000803351.1 LysR family transcriptional regulator -
C6H73_RS08960 1709370..1709432 + 63 WP_010723108.1 membrane protein YoeI -
C6H73_RS08965 1709422..1710780 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C6H73_RS08970 1710959..1712017 + 1059 WP_000492346.1 transport protein YeeE -
C6H73_RS08975 1712031..1712258 + 228 WP_000985280.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T97648 WP_000767829.1 NZ_CP027219:1707019-1707273 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T97648 NZ_CP027219:1707019-1707273 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT97648 WP_001259255.1 NZ_CP027219:1706771-1707022 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT97648 NZ_CP027219:1706771-1707022 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References