Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1885175..1885677 Replicon chromosome
Accession NZ_CP027205
Organism Escherichia coli strain WCHEC025943

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CXB56_RS12590 Protein ID WP_000767829.1
Coordinates 1885423..1885677 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CXB56_RS12585 Protein ID WP_001259255.1
Coordinates 1885175..1885426 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXB56_RS12555 1880352..1881419 - 1068 WP_000080060.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CXB56_RS12560 1881419..1882489 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
CXB56_RS12565 1882486..1883790 - 1305 WP_001317939.1 histidinol dehydrogenase -
CXB56_RS12570 1883796..1884695 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CXB56_RS12580 1884841..1884891 - 51 WP_001364200.1 his operon leader peptide -
CXB56_RS12585 1885175..1885426 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CXB56_RS12590 1885423..1885677 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CXB56_RS12595 1885760..1886584 + 825 WP_000754758.1 SDR family oxidoreductase -
CXB56_RS12600 1886630..1887559 + 930 WP_000803351.1 LysR family transcriptional regulator -
CXB56_RS12605 1887774..1887836 + 63 WP_010723108.1 membrane protein YoeI -
CXB56_RS12610 1887826..1889184 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CXB56_RS12615 1889363..1890421 + 1059 WP_000492346.1 transport protein YeeE -
CXB56_RS12620 1890435..1890662 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T97620 WP_000767829.1 NZ_CP027205:1885423-1885677 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T97620 NZ_CP027205:1885423-1885677 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT97620 WP_001259255.1 NZ_CP027205:1885175-1885426 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT97620 NZ_CP027205:1885175-1885426 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References