Detailed information of TA system TA097578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 60474..61125
• Replicon: plasmid unnamed2
• Accession: NZ_CP027180
• Organism: Acinetobacter baumannii strain AR_0070

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: CSB70_RS20780
• Protein ID: WP_000269904.1
• Coordinates: 60474..60830 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSB70_RS20785
• Protein ID: WP_001129974.1
• Coordinates: 60823..61125 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB70_RS20760	57218..58153	+	936	WP_001292329.1	replication initiation protein RepM	-
CSB70_RS20765	58231..58749	+	519	WP_000839337.1	plasmid replication DNA-binding protein	-
CSB70_RS20770	58989..59420	-	432	WP_000042163.1	MarR family transcriptional regulator	-
CSB70_RS20775	59427..59855	-	429	WP_000105988.1	organic hydroperoxide resistance protein	-
CSB70_RS20780	60474..60830	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSB70_RS20785	60823..61125	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
CSB70_RS20790	61118..61327	+	210	WP_000069474.1	hypothetical protein	-
CSB70_RS20795	61661..61876	+	216	WP_000071892.1	cold-shock protein	-
CSB70_RS20800	61974..62201	+	228	WP_000921865.1	hypothetical protein	-
CSB70_RS20805	62268..62465	+	198	WP_000476222.1	hypothetical protein	-
CSB70_RS20810	62652..62972	+	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CSB70_RS20815	62965..63237	+	273	WP_000369782.1	helix-turn-helix domain-containing protein	-
CSB70_RS20820	63809..64237	+	429	WP_000105988.1	organic hydroperoxide resistance protein	-
CSB70_RS20825	64244..64675	+	432	WP_000042163.1	MarR family transcriptional regulator	-
CSB70_RS20830	64915..65433	-	519	WP_000839337.1	plasmid replication DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..73018	73018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T97578 WP_000269904.1 NZ_CP027180:60474-60830 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T97578 NZ_CP027180:60474-60830 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAATGAATGGCTTGAACAGCAAGATGAAGCAACACAAGAGAAAGTCTTAGC
TGCATTGGTTGTTCTACAACAGCAAGGGCCTAGTTTAGGTCGTCCTTTAGTAGATACCGTCTACGATTCTAAATTTACCA
ATATGAAGGAACTACGTGTTCAGCATCGTGGTAAACCGTTAAGGGCATTTTTTGCATTCGATCCCCTAAGACAAGCGATT
GTGCTGTGTATTGGTGATAAGAGTAATAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATGAGCAATACGCACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT97578 WP_001129974.1 NZ_CP027180:60823-61125 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT97578 NZ_CP027180:60823-61125 [Acinetobacter baumannii]
ATGGCTAAGAGCTTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTTTTAGAAAGTCAGCTTCACCTTATCCGAGAAGAGTTAGAAATTTCCCAGAAAGAACTAGCTGCAACCCTTGGAATTA
AACAGCCGTCATTATCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGTGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGGTTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1