Detailed information of TA system TA097576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 49993..50578
• Replicon: plasmid unnamed2
• Accession: NZ_CP027180
• Organism: Acinetobacter baumannii strain AR_0070

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8ZPD7
• Locus tag: CSB70_RS20705
• Protein ID: WP_000897309.1
• Coordinates: 50258..50578 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSB70_RS20700
• Protein ID: WP_000369782.1
• Coordinates: 49993..50265 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB70_RS20680	46176..47344	-	1169	Protein_70	MobA/MobL family protein	-
CSB70_RS20685	47649..48410	+	762	WP_000425105.1	hypothetical protein	-
CSB70_RS20690	48557..48988	-	432	WP_000042163.1	MarR family transcriptional regulator	-
CSB70_RS20695	48995..49423	-	429	WP_000105988.1	organic hydroperoxide resistance protein	-
CSB70_RS20700	49993..50265	-	273	WP_000369782.1	helix-turn-helix domain-containing protein	Antitoxin
CSB70_RS20705	50258..50578	-	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSB70_RS20710	50765..50962	-	198	WP_000476222.1	hypothetical protein	-
CSB70_RS20715	51029..51256	-	228	WP_000921865.1	hypothetical protein	-
CSB70_RS20720	51354..51569	-	216	WP_000071892.1	cold-shock protein	-
CSB70_RS20725	51902..52110	-	209	Protein_79	hypothetical protein	-
CSB70_RS20730	52103..52405	-	303	WP_001129974.1	XRE family transcriptional regulator	-
CSB70_RS20735	52398..52754	-	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CSB70_RS20740	53373..53801	+	429	WP_000105988.1	organic hydroperoxide resistance protein	-
CSB70_RS20745	53808..54239	+	432	WP_000042163.1	MarR family transcriptional regulator	-
CSB70_RS20750	54386..55147	-	762	WP_000425105.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..73018	73018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12209.01 Da
• Isoelectric Point: 7.9835

>T97576 WP_000897309.1 NZ_CP027180:c50578-50258 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T97576 NZ_CP027180:c50578-50258 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATTTTTACTAAGCAAATCAAAGAGCTTGTGAGTGATGAAGAGTATCGTCAGCTCCAGCA
AGATCTCTTGGTACAGCCTGATAAGGGCGACTTAATCAAGAATGGTGGTGGAATTCGTAAAGTACGTTGTGCTCAGGGCA
ATAAAGGCAAAAGTGGCGGTATCCGTGTGATCTATTATTGGGTTACAGAAGATGATCAGATCTTTTTCTTGGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAAACCTCGATTCTACGTCAATTAGTAAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10064.50 Da
• Isoelectric Point: 7.2045

>AT97576 WP_000369782.1 NZ_CP027180:c50265-49993 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRNEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPGLI

Nucleotide

• Length: 273 bp

>AT97576 NZ_CP027180:c50265-49993 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACCTGGTTGCTTCAATCAAAGAAGCTGGTGCCATCAAGCGTAATGAGATAAAGGCAAG
TCGAGTCACAGAGCTTGAATTACCGGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCTGAATTTGCTGCAC
GTTTGCATATCAGTGCAAGAACCCTGCAAAACTGGGAACAAGGCCGTCGCTATCCGACTGGCCCAGCAGCAACTTTAATC
CGTATTCTTGATGCCCATCCAGGTCTGATTTAA

Structures

Toxin

• AlphaFold DB: A0A855WJQ3