Detailed information of TA system TA097575

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 41785..42370
• Replicon: plasmid unnamed2
• Accession: NZ_CP027180
• Organism: Acinetobacter baumannii strain AR_0070

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8ZPD7
• Locus tag: CSB70_RS20650
• Protein ID: WP_000897309.1
• Coordinates: 41785..42105 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSB70_RS20655
• Protein ID: WP_000369782.1
• Coordinates: 42098..42370 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB70_RS20605	37214..37975	+	762	WP_000425105.1	hypothetical protein	-
CSB70_RS20610	38122..38553	-	432	WP_000042163.1	MarR family transcriptional regulator	-
CSB70_RS20615	38560..38988	-	429	WP_000105988.1	organic hydroperoxide resistance protein	-
CSB70_RS20620	39607..39963	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CSB70_RS20625	39956..40258	+	303	WP_001129974.1	XRE family transcriptional regulator	-
CSB70_RS20630	40251..40460	+	210	WP_000069474.1	hypothetical protein	-
CSB70_RS20635	40794..41009	+	216	WP_000071892.1	cold-shock protein	-
CSB70_RS20640	41107..41334	+	228	WP_000921865.1	hypothetical protein	-
CSB70_RS20645	41401..41598	+	198	WP_000476222.1	hypothetical protein	-
CSB70_RS20650	41785..42105	+	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSB70_RS20655	42098..42370	+	273	WP_000369782.1	helix-turn-helix domain-containing protein	Antitoxin
CSB70_RS20660	42942..43370	+	429	WP_000105988.1	organic hydroperoxide resistance protein	-
CSB70_RS20665	43377..43808	+	432	WP_000042163.1	MarR family transcriptional regulator	-
CSB70_RS20670	44048..44566	-	519	WP_000839337.1	plasmid replication DNA-binding protein	-
CSB70_RS20675	44644..45579	-	936	WP_001292329.1	replication initiation protein RepM	-
CSB70_RS20680	46176..47344	-	1169	Protein_70	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..73018	73018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12209.01 Da
• Isoelectric Point: 7.9835

>T97575 WP_000897309.1 NZ_CP027180:41785-42105 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T97575 NZ_CP027180:41785-42105 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATTTTTACTAAGCAAATCAAAGAGCTTGTGAGTGATGAAGAGTATCGTCAGCTCCAGCA
AGATCTCTTGGTACAGCCTGATAAGGGCGACTTAATCAAGAATGGTGGTGGAATTCGTAAAGTACGTTGTGCTCAGGGCA
ATAAAGGCAAAAGTGGCGGTATCCGTGTGATCTATTATTGGGTTACAGAAGATGATCAGATCTTTTTCTTGGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAAACCTCGATTCTACGTCAATTAGTAAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10064.50 Da
• Isoelectric Point: 7.2045

>AT97575 WP_000369782.1 NZ_CP027180:42098-42370 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRNEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPGLI

Nucleotide

• Length: 273 bp

>AT97575 NZ_CP027180:42098-42370 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACCTGGTTGCTTCAATCAAAGAAGCTGGTGCCATCAAGCGTAATGAGATAAAGGCAAG
TCGAGTCACAGAGCTTGAATTACCGGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCTGAATTTGCTGCAC
GTTTGCATATCAGTGCAAGAACCCTGCAAAACTGGGAACAAGGCCGTCGCTATCCGACTGGCCCAGCAGCAACTTTAATC
CGTATTCTTGATGCCCATCCAGGTCTGATTTAA

Structures

Toxin

• AlphaFold DB: A0A855WJQ3