Detailed information of TA system TA097574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 39607..40258
• Replicon: plasmid unnamed2
• Accession: NZ_CP027180
• Organism: Acinetobacter baumannii strain AR_0070

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: CSB70_RS20620
• Protein ID: WP_000269904.1
• Coordinates: 39607..39963 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSB70_RS20625
• Protein ID: WP_001129974.1
• Coordinates: 39956..40258 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB70_RS20600	35740..36909	-	1170	WP_002134206.1	MobA/MobL family protein	-
CSB70_RS20605	37214..37975	+	762	WP_000425105.1	hypothetical protein	-
CSB70_RS20610	38122..38553	-	432	WP_000042163.1	MarR family transcriptional regulator	-
CSB70_RS20615	38560..38988	-	429	WP_000105988.1	organic hydroperoxide resistance protein	-
CSB70_RS20620	39607..39963	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSB70_RS20625	39956..40258	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
CSB70_RS20630	40251..40460	+	210	WP_000069474.1	hypothetical protein	-
CSB70_RS20635	40794..41009	+	216	WP_000071892.1	cold-shock protein	-
CSB70_RS20640	41107..41334	+	228	WP_000921865.1	hypothetical protein	-
CSB70_RS20645	41401..41598	+	198	WP_000476222.1	hypothetical protein	-
CSB70_RS20650	41785..42105	+	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CSB70_RS20655	42098..42370	+	273	WP_000369782.1	helix-turn-helix domain-containing protein	-
CSB70_RS20660	42942..43370	+	429	WP_000105988.1	organic hydroperoxide resistance protein	-
CSB70_RS20665	43377..43808	+	432	WP_000042163.1	MarR family transcriptional regulator	-
CSB70_RS20670	44048..44566	-	519	WP_000839337.1	plasmid replication DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..73018	73018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T97574 WP_000269904.1 NZ_CP027180:39607-39963 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T97574 NZ_CP027180:39607-39963 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAATGAATGGCTTGAACAGCAAGATGAAGCAACACAAGAGAAAGTCTTAGC
TGCATTGGTTGTTCTACAACAGCAAGGGCCTAGTTTAGGTCGTCCTTTAGTAGATACCGTCTACGATTCTAAATTTACCA
ATATGAAGGAACTACGTGTTCAGCATCGTGGTAAACCGTTAAGGGCATTTTTTGCATTCGATCCCCTAAGACAAGCGATT
GTGCTGTGTATTGGTGATAAGAGTAATAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATGAGCAATACGCACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT97574 WP_001129974.1 NZ_CP027180:39956-40258 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT97574 NZ_CP027180:39956-40258 [Acinetobacter baumannii]
ATGGCTAAGAGCTTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTTTTAGAAAGTCAGCTTCACCTTATCCGAGAAGAGTTAGAAATTTCCCAGAAAGAACTAGCTGCAACCCTTGGAATTA
AACAGCCGTCATTATCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGTGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGGTTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1