Detailed information of TA system TA097572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 29124..29709
• Replicon: plasmid unnamed2
• Accession: NZ_CP027180
• Organism: Acinetobacter baumannii strain AR_0070

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8ZPD7
• Locus tag: CSB70_RS20545
• Protein ID: WP_000897309.1
• Coordinates: 29389..29709 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSB70_RS20540
• Protein ID: WP_000369782.1
• Coordinates: 29124..29396 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB70_RS20520	25304..26473	-	1170	WP_002134206.1	MobA/MobL family protein	-
CSB70_RS20525	26778..27539	+	762	WP_000425105.1	hypothetical protein	-
CSB70_RS20530	27686..28117	-	432	WP_000042163.1	MarR family transcriptional regulator	-
CSB70_RS20535	28124..28552	-	429	WP_000105988.1	organic hydroperoxide resistance protein	-
CSB70_RS20540	29124..29396	-	273	WP_000369782.1	helix-turn-helix domain-containing protein	Antitoxin
CSB70_RS20545	29389..29709	-	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSB70_RS20550	29896..30093	-	198	WP_000476222.1	hypothetical protein	-
CSB70_RS20555	30160..30387	-	228	WP_000921865.1	hypothetical protein	-
CSB70_RS20560	30485..30700	-	216	WP_000071892.1	cold-shock protein	-
CSB70_RS20565	31034..31243	-	210	WP_000069474.1	hypothetical protein	-
CSB70_RS20570	31236..31538	-	303	WP_001129974.1	XRE family transcriptional regulator	-
CSB70_RS20575	31531..31887	-	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CSB70_RS20580	32506..32934	+	429	WP_000105988.1	organic hydroperoxide resistance protein	-
CSB70_RS20585	32941..33372	+	432	WP_000042163.1	MarR family transcriptional regulator	-
CSB70_RS20590	33612..34130	-	519	WP_000839337.1	plasmid replication DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..73018	73018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12209.01 Da
• Isoelectric Point: 7.9835

>T97572 WP_000897309.1 NZ_CP027180:c29709-29389 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T97572 NZ_CP027180:c29709-29389 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATTTTTACTAAGCAAATCAAAGAGCTTGTGAGTGATGAAGAGTATCGTCAGCTCCAGCA
AGATCTCTTGGTACAGCCTGATAAGGGCGACTTAATCAAGAATGGTGGTGGAATTCGTAAAGTACGTTGTGCTCAGGGCA
ATAAAGGCAAAAGTGGCGGTATCCGTGTGATCTATTATTGGGTTACAGAAGATGATCAGATCTTTTTCTTGGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAAACCTCGATTCTACGTCAATTAGTAAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10064.50 Da
• Isoelectric Point: 7.2045

>AT97572 WP_000369782.1 NZ_CP027180:c29396-29124 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRNEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPGLI

Nucleotide

• Length: 273 bp

>AT97572 NZ_CP027180:c29396-29124 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACCTGGTTGCTTCAATCAAAGAAGCTGGTGCCATCAAGCGTAATGAGATAAAGGCAAG
TCGAGTCACAGAGCTTGAATTACCGGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCTGAATTTGCTGCAC
GTTTGCATATCAGTGCAAGAACCCTGCAAAACTGGGAACAAGGCCGTCGCTATCCGACTGGCCCAGCAGCAACTTTAATC
CGTATTCTTGATGCCCATCCAGGTCTGATTTAA

Structures

Toxin

• AlphaFold DB: A0A855WJQ3