Detailed information of TA system TA097569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 8313..8964
• Replicon: plasmid unnamed2
• Accession: NZ_CP027180
• Organism: Acinetobacter baumannii strain AR_0070

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: CSB70_RS20380
• Protein ID: WP_000269904.1
• Coordinates: 8313..8669 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSB70_RS20385
• Protein ID: WP_001129974.1
• Coordinates: 8662..8964 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB70_RS20360	4448..5616	-	1169	Protein_6	MobA/MobL family protein	-
CSB70_RS20365	5921..6682	+	762	WP_000425105.1	hypothetical protein	-
CSB70_RS20370	6829..7260	-	432	WP_000042163.1	MarR family transcriptional regulator	-
CSB70_RS20375	7267..7695	-	429	WP_000105988.1	organic hydroperoxide resistance protein	-
CSB70_RS20380	8313..8669	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSB70_RS20385	8662..8964	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
CSB70_RS20390	8957..9166	+	210	WP_000069474.1	hypothetical protein	-
CSB70_RS20395	9499..9714	+	216	WP_000071892.1	cold-shock protein	-
CSB70_RS20400	9810..10037	+	228	WP_000921865.1	hypothetical protein	-
CSB70_RS20405	10104..10301	+	198	WP_000476222.1	hypothetical protein	-
CSB70_RS20410	10488..10808	+	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CSB70_RS20415	10801..11073	+	273	WP_000369782.1	helix-turn-helix domain-containing protein	-
CSB70_RS20420	11645..12073	+	429	WP_000105988.1	organic hydroperoxide resistance protein	-
CSB70_RS20425	12080..12511	+	432	WP_000042163.1	MarR family transcriptional regulator	-
CSB70_RS20430	12657..13418	-	762	WP_000425105.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..73018	73018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T97569 WP_000269904.1 NZ_CP027180:8313-8669 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T97569 NZ_CP027180:8313-8669 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAATGAATGGCTTGAACAGCAAGATGAAGCAACACAAGAGAAAGTCTTAGC
TGCATTGGTTGTTCTACAACAGCAAGGGCCTAGTTTAGGTCGTCCTTTAGTAGATACCGTCTACGATTCTAAATTTACCA
ATATGAAGGAACTACGTGTTCAGCATCGTGGTAAACCGTTAAGGGCATTTTTTGCATTCGATCCCCTAAGACAAGCGATT
GTGCTGTGTATTGGTGATAAGAGTAATAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATGAGCAATACGCACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT97569 WP_001129974.1 NZ_CP027180:8662-8964 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT97569 NZ_CP027180:8662-8964 [Acinetobacter baumannii]
ATGGCTAAGAGCTTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTTTTAGAAAGTCAGCTTCACCTTATCCGAGAAGAGTTAGAAATTTCCCAGAAAGAACTAGCTGCAACCCTTGGAATTA
AACAGCCGTCATTATCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGTGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGGTTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1