Detailed information of TA system TA097559

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4435366..4435961
• Replicon: chromosome
• Accession: NZ_CP027174
• Organism: Pseudomonas aeruginosa strain AR_0230

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: CSB85_RS21310
• Protein ID: WP_003113526.1
• Coordinates: 4435683..4435961 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSB85_RS21305
• Protein ID: WP_003113527.1
• Coordinates: 4435366..4435671 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB85_RS21285	4431073..4431864	+	792	WP_034019065.1	IS21-like element helper ATPase IstB	-
CSB85_RS21290	4432071..4432934	-	864	WP_034019067.1	integrase domain-containing protein	-
CSB85_RS21295	4433537..4434694	-	1158	WP_034019070.1	hypothetical protein	-
CSB85_RS21305	4435366..4435671	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
CSB85_RS21310	4435683..4435961	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSB85_RS21320	4436290..4438518	+	2229	WP_033942306.1	TonB-dependent receptor	-
CSB85_RS21325	4438588..4439235	-	648	WP_003095021.1	carbonate dehydratase	-
CSB85_RS21330	4439297..4440535	-	1239	WP_033942304.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T97559 WP_003113526.1 NZ_CP027174:c4435961-4435683 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T97559 NZ_CP027174:c4435961-4435683 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT97559 WP_003113527.1 NZ_CP027174:c4435671-4435366 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT97559 NZ_CP027174:c4435671-4435366 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3