Detailed information of TA system TA097546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1916673..1917354
• Replicon: chromosome
• Accession: NZ_CP027172
• Organism: Pseudomonas aeruginosa strain AR_0353

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CSB90_RS09050
• Protein ID: WP_015503432.1
• Coordinates: 1916673..1917038 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSB90_RS09055
• Protein ID: WP_004364117.1
• Coordinates: 1917031..1917354 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB90_RS09020	1912669..1913103	-	435	WP_003158601.1	RidA family protein	-
CSB90_RS09025	1913324..1914277	+	954	WP_003085661.1	LysR family transcriptional regulator	-
CSB90_RS09030	1914250..1915134	-	885	WP_004364122.1	LysR family transcriptional regulator	-
CSB90_RS09035	1915235..1915660	+	426	WP_003116492.1	VOC family protein	-
CSB90_RS09040	1915691..1915963	-	273	WP_004364121.1	hypothetical protein	-
CSB90_RS09045	1916170..1916409	+	240	WP_044069615.1	hypothetical protein	-
CSB90_RS09050	1916673..1917038	+	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSB90_RS09055	1917031..1917354	+	324	WP_004364117.1	XRE family transcriptional regulator	Antitoxin
CSB90_RS09060	1917470..1917589	-	120	Protein_1753	IS5/IS1182 family transposase	-
CSB90_RS09065	1917644..1917976	+	333	WP_016561578.1	hypothetical protein	-
CSB90_RS09070	1917976..1918251	+	276	WP_004364115.1	hypothetical protein	-
CSB90_RS09075	1918426..1918980	+	555	WP_074197746.1	tyrosine-type recombinase/integrase	-
CSB90_RS09080	1919068..1920230	+	1163	WP_087920907.1	IS3 family transposase	-
CSB90_RS09090	1921001..1921578	-	578	Protein_1758	plasmid pRiA4b ORF-3 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1915235..1950797	35562

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-86)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T97546 WP_015503432.1 NZ_CP027172:1916673-1917038 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T97546 NZ_CP027172:1916673-1917038 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT97546 WP_004364117.1 NZ_CP027172:1917031-1917354 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GHLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT97546 NZ_CP027172:1917031-1917354 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCATCTGGAAGTGATTGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCTGATATCGAAGAGGCGGGCGC
CTAA