Detailed information of TA system TA097543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1191147..1191742
• Replicon: chromosome
• Accession: NZ_CP027172
• Organism: Pseudomonas aeruginosa strain AR_0353

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: CSB90_RS05540
• Protein ID: WP_003113526.1
• Coordinates: 1191147..1191425 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSB90_RS05545
• Protein ID: WP_003133769.1
• Coordinates: 1191437..1191742 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB90_RS05520	1186572..1187810	+	1239	WP_003095023.1	C69 family dipeptidase	-
CSB90_RS05525	1187872..1188519	+	648	WP_003095021.1	carbonate dehydratase	-
CSB90_RS05530	1188590..1190818	-	2229	WP_003133778.1	TonB-dependent receptor	-
CSB90_RS05540	1191147..1191425	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSB90_RS05545	1191437..1191742	+	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
CSB90_RS05550	1192185..1193177	+	993	WP_003133766.1	hypothetical protein	-
CSB90_RS05555	1193261..1194526	-	1266	WP_023094906.1	nucleotidyltransferase	-
CSB90_RS35570	1194523..1195113	-	591	WP_071536478.1	SLATT domain-containing protein	-
CSB90_RS05560	1195207..1195542	-	336	WP_023094905.1	hypothetical protein	-
CSB90_RS05570	1195982..1196254	+	273	WP_004352675.1	hypothetical protein	-
CSB90_RS05575	1196430..1196720	+	291	WP_031757220.1	DUF5447 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T97543 WP_003113526.1 NZ_CP027172:1191147-1191425 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T97543 NZ_CP027172:1191147-1191425 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT97543 WP_003133769.1 NZ_CP027172:1191437-1191742 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT97543 NZ_CP027172:1191437-1191742 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTACGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCCCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3