Detailed information of TA system TA097532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2822646..2823241
• Replicon: chromosome
• Accession: NZ_CP027166
• Organism: Pseudomonas aeruginosa strain AR_0357

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: CSB94_RS13570
• Protein ID: WP_003113526.1
• Coordinates: 2822646..2822924 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSB94_RS13575
• Protein ID: WP_003133769.1
• Coordinates: 2822936..2823241 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB94_RS13550	2818071..2819309	+	1239	WP_003095023.1	C69 family dipeptidase	-
CSB94_RS13555	2819371..2820018	+	648	WP_003095021.1	carbonate dehydratase	-
CSB94_RS13560	2820089..2822317	-	2229	WP_023104079.1	TonB-dependent receptor	-
CSB94_RS13570	2822646..2822924	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSB94_RS13575	2822936..2823241	+	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
CSB94_RS13585	2823913..2825055	+	1143	WP_023098851.1	hypothetical protein	-
CSB94_RS13590	2825656..2826519	+	864	WP_023098850.1	integrase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T97532 WP_003113526.1 NZ_CP027166:2822646-2822924 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T97532 NZ_CP027166:2822646-2822924 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT97532 WP_003133769.1 NZ_CP027166:2822936-2823241 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT97532 NZ_CP027166:2822936-2823241 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3