Detailed information of TA system TA097529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4213503..4214098
• Replicon: chromosome
• Accession: NZ_CP027165
• Organism: Pseudomonas aeruginosa strain AR_0360

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: CSB97_RS19960
• Protein ID: WP_003113526.1
• Coordinates: 4213820..4214098 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSB97_RS19955
• Protein ID: WP_003113527.1
• Coordinates: 4213503..4213808 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB97_RS19920	4208966..4209256	-	291	WP_023083237.1	DUF5447 family protein	-
CSB97_RS19930	4209468..4209740	-	273	WP_003115921.1	hypothetical protein	-
CSB97_RS19935	4209850..4210116	+	267	WP_016852153.1	hypothetical protein	-
CSB97_RS19940	4210239..4211084	+	846	WP_196645791.1	helix-turn-helix domain-containing protein	-
CSB97_RS19945	4211059..4212597	+	1539	WP_079759796.1	GNAT family N-acetyltransferase	-
CSB97_RS19950	4212609..4213136	-	528	WP_071535723.1	AAA family ATPase	-
CSB97_RS19955	4213503..4213808	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
CSB97_RS19960	4213820..4214098	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSB97_RS19970	4214427..4216655	+	2229	WP_033942306.1	TonB-dependent receptor	-
CSB97_RS19975	4216725..4217372	-	648	WP_003095021.1	carbonate dehydratase	-
CSB97_RS19980	4217434..4218672	-	1239	WP_033970863.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T97529 WP_003113526.1 NZ_CP027165:c4214098-4213820 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T97529 NZ_CP027165:c4214098-4213820 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT97529 WP_003113527.1 NZ_CP027165:c4213808-4213503 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT97529 NZ_CP027165:c4213808-4213503 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3