Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 2585610..2586196 Replicon chromosome
Accession NZ_CP027146
Organism Klebsiella pneumoniae strain AR_0363

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag CSC00_RS12935 Protein ID WP_002920800.1
Coordinates 2585828..2586196 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag CSC00_RS12930 Protein ID WP_002920802.1
Coordinates 2585610..2585831 (+) Length 74 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC00_RS12910 2581767..2582693 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
CSC00_RS12915 2582690..2583967 + 1278 WP_002920806.1 branched chain amino acid ABC transporter permease LivM -
CSC00_RS12920 2583964..2584731 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
CSC00_RS12925 2584733..2585446 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
CSC00_RS12930 2585610..2585831 + 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CSC00_RS12935 2585828..2586196 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CSC00_RS12940 2586469..2587785 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
CSC00_RS12945 2587892..2588779 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
CSC00_RS12950 2588776..2589621 + 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
CSC00_RS12955 2589623..2590693 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2575377..2591430 16053


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(13-86)

Antitoxin

(1-49)


Sequences


Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T97488 WP_002920800.1 NZ_CP027146:2585828-2586196 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T97488 NZ_CP027146:2585828-2586196 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT97488 WP_002920802.1 NZ_CP027146:2585610-2585831 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT97488 NZ_CP027146:2585610-2585831 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1


Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References