Detailed information of TA system TA097479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3948200..3948930
• Replicon: chromosome
• Accession: NZ_CP027142
• Organism: Enterobacter hormaechei subsp. hoffmannii strain AR_0365

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: CSC02_RS19580
• Protein ID: WP_023302825.1
• Coordinates: 3948200..3948514 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSC02_RS19585
• Protein ID: WP_139156973.1
• Coordinates: 3948511..3948930 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC02_RS19555	3943866..3944951	+	1086	Protein_3726	cellulase family glycosylhydrolase	-
CSC02_RS19560	3944857..3946041	-	1185	WP_023305143.1	multidrug efflux MFS transporter EmrD	-
CSC02_RS19565	3946217..3947050	-	834	WP_023305144.1	DMT family transporter	-
CSC02_RS19570	3947113..3947559	-	447	WP_023298965.1	GNAT family N-acetyltransferase	-
CSC02_RS19575	3947624..3947713	-	90	WP_022646551.1	type I toxin-antitoxin system toxin TisB	-
CSC02_RS19580	3948200..3948514	+	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
CSC02_RS19585	3948511..3948930	+	420	WP_139156973.1	helix-turn-helix domain-containing protein	Antitoxin
CSC02_RS19590	3949077..3949175	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
CSC02_RS19595	3949282..3950970	+	1689	WP_032676730.1	acetolactate synthase large subunit	-
CSC02_RS19600	3950974..3951261	+	288	WP_010426528.1	acetolactate synthase small subunit	-
CSC02_RS19605	3951387..3951980	+	594	WP_022646555.1	transcriptional regulator UhpA	-
CSC02_RS19610	3951977..3953482	+	1506	WP_023305147.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-137)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T97479 WP_023302825.1 NZ_CP027142:3948200-3948514 [Enterobacter hormaechei subsp. hoffmannii]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T97479 NZ_CP027142:3948200-3948514 [Enterobacter hormaechei subsp. hoffmannii]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAACACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15495.15 Da
• Isoelectric Point: 5.6761

>AT97479 WP_139156973.1 NZ_CP027142:3948511-3948930 [Enterobacter hormaechei subsp. hoffmannii]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYTLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAVRFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT97479 NZ_CP027142:3948511-3948930 [Enterobacter hormaechei subsp. hoffmannii]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCACGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTTT
GCGCCAGAATTAGCCATTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCTGTTCCCGTCGGA
ATTGCAGTTTTGAGAACCTTGATGGATCAATACACTCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACTCTGAACCATATTAAAAAGCTGGCGGTCAGATTTGGGGTATCAC
CTGCGCTATTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A837FIL2