Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2728291..2729018 Replicon chromosome
Accession NZ_CP027140
Organism Escherichia coli strain AR_0369

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag CSC06_RS16145 Protein ID WP_000550189.1
Coordinates 2728291..2728605 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag CSC06_RS16150 Protein ID WP_001514564.1
Coordinates 2728602..2729018 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC06_RS16120 2724457..2725443 - 987 WP_001301393.1 Gfo/Idh/MocA family oxidoreductase -
CSC06_RS16125 2725522..2726205 - 684 WP_001183046.1 vancomycin high temperature exclusion protein -
CSC06_RS16130 2726282..2726785 - 504 WP_001295542.1 M48 family metallopeptidase -
CSC06_RS16135 2726870..2728006 + 1137 WP_105394284.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
CSC06_RS16145 2728291..2728605 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
CSC06_RS16150 2728602..2729018 + 417 WP_001514564.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
CSC06_RS16155 2729063..2731081 - 2019 WP_105394285.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
CSC06_RS16160 2731507..2733858 - 2352 WP_105394286.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-136)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T97449 WP_000550189.1 NZ_CP027140:2728291-2728605 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T97449 NZ_CP027140:2728291-2728605 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15025.44 Da        Isoelectric Point: 4.4547

>AT97449 WP_001514564.1 NZ_CP027140:2728602-2729018 [Escherichia coli]
MITIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT97449 NZ_CP027140:2728602-2729018 [Escherichia coli]
ATGATTACGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References