Detailed information of TA system TA097448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2684915..2685714
• Replicon: chromosome
• Accession: NZ_CP027140
• Organism: Escherichia coli strain AR_0369

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: S1NYM6
• Locus tag: CSC06_RS15895
• Protein ID: WP_000347251.1
• Coordinates: 2684915..2685379 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: CSC06_RS15900
• Protein ID: WP_001307405.1
• Coordinates: 2685379..2685714 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC06_RS15860	2679916..2680350	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
CSC06_RS15865	2680368..2681246	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
CSC06_RS15870	2681236..2682015	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
CSC06_RS15875	2682026..2682499	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
CSC06_RS15880	2682522..2683802	-	1281	WP_000681905.1	tagatose-bisphosphate aldolase subunit KbaZ	-
CSC06_RS15890	2684051..2684860	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
CSC06_RS15895	2684915..2685379	-	465	WP_000347251.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
CSC06_RS15900	2685379..2685714	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
CSC06_RS15905	2685863..2687434	-	1572	WP_001273753.1	galactarate dehydratase	-
CSC06_RS15910	2687809..2689143	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
CSC06_RS15915	2689159..2689929	+	771	WP_001058209.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17777.14 Da
• Isoelectric Point: 9.4947

>T97448 WP_000347251.1 NZ_CP027140:c2685379-2684915 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T97448 NZ_CP027140:c2685379-2684915 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT97448 WP_001307405.1 NZ_CP027140:c2685714-2685379 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT97448 NZ_CP027140:c2685714-2685379 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829CJ20

Antitoxin

• AlphaFold DB: A0A0E0XVC7