Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1111786..1112327 Replicon chromosome
Accession NZ_CP027140
Organism Escherichia coli strain AR_0369

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag CSC06_RS07915 Protein ID WP_000615979.1
Coordinates 1112049..1112327 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CSC06_RS07910 Protein ID WP_000729704.1
Coordinates 1111786..1112046 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC06_RS07890 1107469..1108254 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
CSC06_RS07895 1108226..1109938 + 1713 Protein_1079 flagellar biosynthesis protein FlhA -
CSC06_RS07900 1110154..1110651 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
CSC06_RS07905 1110827..1111600 - 774 WP_123907333.1 C40 family peptidase -
CSC06_RS07910 1111786..1112046 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CSC06_RS07915 1112049..1112327 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CSC06_RS07920 1112483..1113223 + 741 WP_001225679.1 murein L,D-transpeptidase -
CSC06_RS07925 1113194..1113961 - 768 WP_000333379.1 class II glutamine amidotransferase -
CSC06_RS07930 1114167..1114745 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / tssM / tssA / hcp1/tssD1 1072495..1129686 57191


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T97446 WP_000615979.1 NZ_CP027140:1112049-1112327 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T97446 NZ_CP027140:1112049-1112327 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT97446 WP_000729704.1 NZ_CP027140:1111786-1112046 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT97446 NZ_CP027140:1111786-1112046 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References