Detailed information of TA system TA097445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1105597..1106291
• Replicon: chromosome
• Accession: NZ_CP027140
• Organism: Escherichia coli strain AR_0369

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: CSC06_RS07875
• Protein ID: WP_001263493.1
• Coordinates: 1105597..1105995 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: CSC06_RS07880
• Protein ID: WP_000554757.1
• Coordinates: 1105998..1106291 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC06_RS07845	1100597..1101841	-	1245	WP_000189541.1	esterase FrsA	-
-	1101257..1101337	-	81	NuclAT_11	-	-
-	1101257..1101337	-	81	NuclAT_11	-	-
-	1101257..1101337	-	81	NuclAT_11	-	-
-	1101257..1101337	-	81	NuclAT_11	-	-
CSC06_RS07850	1101933..1102391	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CSC06_RS07855	1102652..1104109	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
CSC06_RS07860	1104166..1104687	-	522	Protein_1072	peptide chain release factor H	-
CSC06_RS07865	1104686..1104889	-	204	Protein_1073	RNA ligase RtcB family protein	-
CSC06_RS07870	1105135..1105587	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
CSC06_RS07875	1105597..1105995	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CSC06_RS07880	1105998..1106291	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CSC06_RS07885	1106343..1107398	-	1056	WP_001226164.1	DNA polymerase IV	-
CSC06_RS07890	1107469..1108254	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
CSC06_RS07895	1108226..1109938	+	1713	Protein_1079	flagellar biosynthesis protein FlhA	-
CSC06_RS07900	1110154..1110651	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / tssM / tssA / hcp1/tssD1	1072495..1129686	57191

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T97445 WP_001263493.1 NZ_CP027140:c1105995-1105597 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T97445 NZ_CP027140:c1105995-1105597 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT97445 WP_000554757.1 NZ_CP027140:c1106291-1105998 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT97445 NZ_CP027140:c1106291-1105998 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6