Detailed information of TA system TA097434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4480447..4481246
• Replicon: chromosome
• Accession: NZ_CP027134
• Organism: Escherichia coli strain AR_0372

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: D3GWU5
• Locus tag: CSC09_RS24910
• Protein ID: WP_000347272.1
• Coordinates: 4480782..4481246 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: CSC09_RS24905
• Protein ID: WP_001307405.1
• Coordinates: 4480447..4480782 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC09_RS24890	4476232..4477002	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
CSC09_RS24895	4477018..4478352	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
CSC09_RS24900	4478727..4480298	+	1572	WP_001273738.1	galactarate dehydratase	-
CSC09_RS24905	4480447..4480782	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
CSC09_RS24910	4480782..4481246	+	465	WP_000347272.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
CSC09_RS24915	4481301..4482110	-	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
CSC09_RS24925	4482359..4483639	+	1281	WP_001521382.1	tagatose-bisphosphate aldolase subunit KbaZ	-
CSC09_RS24930	4483662..4484135	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
CSC09_RS24935	4484146..4484925	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
CSC09_RS24940	4484915..4485793	+	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
CSC09_RS24945	4485811..4486245	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17832.26 Da
• Isoelectric Point: 9.6924

>T97434 WP_000347272.1 NZ_CP027134:4480782-4481246 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Nucleotide

• Length: 465 bp

>T97434 NZ_CP027134:4480782-4481246 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT97434 WP_001307405.1 NZ_CP027134:4480447-4480782 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT97434 NZ_CP027134:4480447-4480782 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829KUD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7