Detailed information of TA system TA097414

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2303078..2303449
• Replicon: chromosome
• Accession: NZ_CP027134
• Organism: Escherichia coli strain AR_0372

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: CSC09_RS13570
• Protein ID: WP_001443846.1
• Coordinates: 2303300..2303449 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2303078..2303256 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC09_RS13545	2299033..2300406	+	1374	WP_000123748.1	ATP-dependent RNA helicase DbpA	-
CSC09_RS13550	2300535..2301470	-	936	WP_001523172.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
CSC09_RS13555	2301522..2302757	-	1236	WP_000040839.1	site-specific integrase	-
CSC09_RS13560	2302759..2302974	-	216	WP_000079604.1	excisionase XisR	-
CSC09_RS13565	2303074..2303262	-	189	WP_001302840.1	DUF1187 family protein	-
-	2303078..2303256	+	179	NuclAT_0	-	Antitoxin
-	2303078..2303256	+	179	NuclAT_0	-	Antitoxin
-	2303078..2303256	+	179	NuclAT_0	-	Antitoxin
-	2303078..2303256	+	179	NuclAT_0	-	Antitoxin
CSC09_RS13570	2303300..2303449	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
CSC09_RS13575	2303505..2304314	-	810	WP_000166313.1	recombination protein RecT	-
CSC09_RS13580	2304307..2306907	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
CSC09_RS13585	2307009..2307284	-	276	WP_001344816.1	hypothetical protein	-
CSC09_RS13590	2307359..2307529	-	171	WP_001352098.1	conserved protein, Rac prophage	-
CSC09_RS13595	2307529..2307750	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2296709..2358376	61667

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T97414 WP_001443846.1 NZ_CP027134:c2303449-2303300 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T97414 NZ_CP027134:c2303449-2303300 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT97414 NZ_CP027134:2303078-2303256 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin