Detailed information of TA system TA097407

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1068795..1069489
• Replicon: chromosome
• Accession: NZ_CP027134
• Organism: Escherichia coli strain AR_0372

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: CSC09_RS07255
• Protein ID: WP_001521903.1
• Coordinates: 1069091..1069489 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: CSC09_RS07250
• Protein ID: WP_000554758.1
• Coordinates: 1068795..1069088 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC09_RS07225	1063910..1064659	+	750	WP_016233951.1	C40 family peptidase	-
CSC09_RS07230	1064716..1065069	-	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
CSC09_RS07235	1065148..1066860	-	1713	Protein_971	flagellar biosynthesis protein FlhA	-
CSC09_RS07240	1066832..1067617	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
CSC09_RS07245	1067688..1068743	+	1056	WP_001226177.1	DNA polymerase IV	-
CSC09_RS07250	1068795..1069088	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CSC09_RS07255	1069091..1069489	+	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CSC09_RS07260	1069499..1069951	+	453	WP_023144376.1	GNAT family N-acetyltransferase	-
CSC09_RS07265	1070196..1070402	+	207	Protein_977	RtcB family protein	-
CSC09_RS07270	1070398..1070750	+	353	Protein_978	peptide chain release factor H	-
CSC09_RS07275	1070807..1072264	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
CSC09_RS07280	1072525..1072983	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CSC09_RS07285	1073075..1074319	+	1245	WP_000189541.1	esterase FrsA	-
-	1073579..1073659	+	81	NuclAT_11	-	-
-	1073579..1073659	+	81	NuclAT_11	-	-
-	1073579..1073659	+	81	NuclAT_11	-	-
-	1073579..1073659	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T97407 WP_001521903.1 NZ_CP027134:1069091-1069489 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T97407 NZ_CP027134:1069091-1069489 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT97407 WP_000554758.1 NZ_CP027134:1068795-1069088 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT97407 NZ_CP027134:1068795-1069088 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3