Detailed information of TA system TA097400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 102196..102798
• Replicon: chromosome
• Accession: NZ_CP027134
• Organism: Escherichia coli strain AR_0372

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CSC09_RS02320
• Protein ID: WP_000897305.1
• Coordinates: 102196..102507 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSC09_RS02325
• Protein ID: WP_000356395.1
• Coordinates: 102508..102798 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC09_RS02300	98129..98587	+	459	WP_000526135.1	IS200/IS605 family transposase	-
CSC09_RS02305	99076..100890	+	1815	WP_020233236.1	hypothetical protein	-
CSC09_RS02315	101059..101967	-	909	WP_001306651.1	alpha/beta hydrolase	-
CSC09_RS02320	102196..102507	+	312	WP_000897305.1	hypothetical protein	Toxin
CSC09_RS02325	102508..102798	+	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
CSC09_RS02330	102883..103095	+	213	WP_000197769.1	hypothetical protein	-
CSC09_RS02335	103157..103435	+	279	WP_001306650.1	hypothetical protein	-
CSC09_RS02345	103854..104072	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CSC09_RS02350	104291..104533	+	243	WP_021523315.1	hypothetical protein	-
CSC09_RS02355	104715..105644	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CSC09_RS02360	105641..106276	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CSC09_RS02365	106273..107175	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	98129..98587	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T97400 WP_000897305.1 NZ_CP027134:102196-102507 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T97400 NZ_CP027134:102196-102507 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT97400 WP_000356395.1 NZ_CP027134:102508-102798 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT97400 NZ_CP027134:102508-102798 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7