Detailed information of TA system TA097393

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 121419..121652
• Replicon: plasmid unnamed1
• Accession: NZ_CP027130
• Organism: Escherichia coli strain AR_0372

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CSC09_RS00870
• Protein ID: WP_001312861.1
• Coordinates: 121419..121577 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	121621..121652 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC09_RS00835	116793..117482	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
CSC09_RS00840	117669..118052	-	384	WP_001151524.1	relaxosome protein TraM	-
CSC09_RS00845	118373..118975	+	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
CSC09_RS00850	119272..120093	-	822	WP_001234426.1	DUF945 domain-containing protein	-
CSC09_RS00855	120211..120498	-	288	WP_000107535.1	hypothetical protein	-
CSC09_RS00870	121419..121577	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	121621..121652	-	32	NuclAT_1	-	Antitoxin
-	121621..121652	-	32	NuclAT_1	-	Antitoxin
-	121621..121652	-	32	NuclAT_1	-	Antitoxin
-	121621..121652	-	32	NuclAT_1	-	Antitoxin
-	123094..123291	-	198	NuclAT_0	-	-
-	123094..123291	-	198	NuclAT_0	-	-
-	123094..123291	-	198	NuclAT_0	-	-
-	123094..123291	-	198	NuclAT_0	-	-
CSC09_RS29155	123103..123291	+	189	WP_001299721.1	hypothetical protein	-
CSC09_RS00880	123303..124022	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
CSC09_RS00885	124019..124453	-	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
CSC09_RS00890	124508..124705	-	198	Protein_150	hypothetical protein	-
CSC09_RS00895	124733..124966	-	234	WP_000005990.1	DUF905 family protein	-
CSC09_RS00900	125034..125573	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
CSC09_RS29355	125599..125805	-	207	WP_000275856.1	hypothetical protein	-
CSC09_RS00915	126215..126421	-	207	WP_001774176.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / sitABCD	iutA / iucD / iucC / iucB / iucA	1..170292	170292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T97393 WP_001312861.1 NZ_CP027130:c121577-121419 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T97393 NZ_CP027130:c121577-121419 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 32 bp

>AT97393 NZ_CP027130:c121652-121621 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin