Detailed information of TA system TA097389

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 81352..81606
• Replicon: plasmid unnamed1
• Accession: NZ_CP027130
• Organism: Escherichia coli strain AR_0372

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CSC09_RS00615
• Protein ID: WP_015387399.1
• Coordinates: 81352..81558 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	81545..81606 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC09_RS00580	76599..77510	-	912	WP_000440183.1	carbamate kinase	-
CSC09_RS00585	77521..78741	-	1221	WP_000410951.1	arginine deiminase	-
CSC09_RS00590	79634..80116	+	483	Protein_95	VENN motif pre-toxin domain-containing protein	-
CSC09_RS00595	80062..80508	-	447	Protein_96	incFII family plasmid replication initiator RepA	-
CSC09_RS00600	80501..80575	-	75	WP_032336874.1	RepA leader peptide Tap	-
CSC09_RS00610	80811..81068	-	258	WP_000083833.1	replication regulatory protein RepA	-
CSC09_RS00615	81352..81558	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	81545..81606	+	62	NuclAT_2	-	Antitoxin
-	81545..81606	+	62	NuclAT_2	-	Antitoxin
-	81545..81606	+	62	NuclAT_2	-	Antitoxin
-	81545..81606	+	62	NuclAT_2	-	Antitoxin
CSC09_RS00625	81745..81927	-	183	WP_000968309.1	hypothetical protein	-
CSC09_RS00635	82028..82644	+	617	Protein_101	IS1 family transposase	-
CSC09_RS00640	82682..84253	-	1572	WP_023149734.1	IS66 family transposase	-
CSC09_RS00645	84273..84620	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
CSC09_RS00650	84620..85297	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
CSC09_RS29350	85352..85441	+	90	Protein_105	IS1 family transposase	-
CSC09_RS00665	85742..85954	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / sitABCD	iutA / iucD / iucC / iucB / iucA	1..170292	170292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T97389 WP_015387399.1 NZ_CP027130:c81558-81352 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T97389 NZ_CP027130:c81558-81352 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT97389 NZ_CP027130:81545-81606 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin