Detailed information of TA system TA097385

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 90821..91085
• Replicon: plasmid unnamed2
• Accession: NZ_CP027127
• Organism: Escherichia coli strain AR_0374

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: CSC11_RS25975
• Protein ID: WP_001303307.1
• Coordinates: 90821..90973 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	91023..91085 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC11_RS25940	86104..86766	+	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-
CSC11_RS25945	86838..87047	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
CSC11_RS26545	87439..87615	+	177	WP_001054900.1	hypothetical protein	-
-	88101..88152	+	52	NuclAT_1	-	-
-	88101..88152	+	52	NuclAT_1	-	-
-	88101..88152	+	52	NuclAT_1	-	-
-	88101..88152	+	52	NuclAT_1	-	-
CSC11_RS25960	88336..89358	+	1023	WP_001572805.1	IS21-like element IS100 family transposase	-
CSC11_RS25965	89355..90137	+	783	WP_001300609.1	IS21-like element IS100kyp family helper ATPase IstB	-
CSC11_RS25970	90498..90749	+	252	WP_001291965.1	hypothetical protein	-
CSC11_RS25975	90821..90973	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
-	91023..91085	+	63	NuclAT_0	-	Antitoxin
-	91023..91085	+	63	NuclAT_0	-	Antitoxin
-	91023..91085	+	63	NuclAT_0	-	Antitoxin
-	91023..91085	+	63	NuclAT_0	-	Antitoxin
CSC11_RS25980	91265..92473	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
CSC11_RS25985	92492..93562	+	1071	WP_000151575.1	IncI1-type conjugal transfer protein TrbB	-
CSC11_RS25990	93555..95846	+	2292	WP_001289271.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-2	-	1..98987	98987

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T97385 WP_001303307.1 NZ_CP027127:c90973-90821 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T97385 NZ_CP027127:c90973-90821 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT97385 NZ_CP027127:91023-91085 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin