Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3589860..3590554 Replicon chromosome
Accession NZ_CP027118
Organism Escherichia coli strain 26561

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag CYQ10_RS18510 Protein ID WP_001263489.1
Coordinates 3589860..3590258 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag CYQ10_RS18515 Protein ID WP_000554758.1
Coordinates 3590261..3590554 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3585448..3585528 - 81 NuclAT_12 - -
- 3585448..3585528 - 81 NuclAT_12 - -
- 3585448..3585528 - 81 NuclAT_12 - -
- 3585448..3585528 - 81 NuclAT_12 - -
CYQ10_RS18480 3586124..3586582 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
CYQ10_RS18485 3586843..3588300 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
CYQ10_RS18490 3588357..3588878 - 522 Protein_3367 peptide chain release factor H -
CYQ10_RS18495 3588874..3589080 - 207 Protein_3368 RtcB family protein -
CYQ10_RS18505 3589398..3589850 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
CYQ10_RS18510 3589860..3590258 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CYQ10_RS18515 3590261..3590554 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CYQ10_RS18520 3590606..3591661 - 1056 WP_001226164.1 DNA polymerase IV -
CYQ10_RS18525 3591732..3592517 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
CYQ10_RS18530 3592489..3594195 + 1707 Protein_3374 flagellar biosynthesis protein FlhA -
CYQ10_RS18535 3594424..3594921 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T97351 WP_001263489.1 NZ_CP027118:c3590258-3589860 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T97351 NZ_CP027118:c3590258-3589860 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT97351 WP_000554758.1 NZ_CP027118:c3590554-3590261 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT97351 NZ_CP027118:c3590554-3590261 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References