Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1803712..1804214 Replicon chromosome
Accession NZ_CP027118
Organism Escherichia coli strain 26561

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CYQ10_RS09100 Protein ID WP_000767829.1
Coordinates 1803960..1804214 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CYQ10_RS09095 Protein ID WP_001259255.1
Coordinates 1803712..1803963 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CYQ10_RS09065 1798889..1799956 - 1068 WP_000080055.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CYQ10_RS09070 1799956..1801026 - 1071 WP_000108933.1 histidinol-phosphate transaminase -
CYQ10_RS09075 1801023..1802327 - 1305 WP_001352244.1 histidinol dehydrogenase -
CYQ10_RS09080 1802333..1803232 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CYQ10_RS09090 1803378..1803428 - 51 WP_001364200.1 his operon leader peptide -
CYQ10_RS09095 1803712..1803963 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CYQ10_RS09100 1803960..1804214 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CYQ10_RS09105 1804297..1805121 + 825 WP_000754737.1 SDR family oxidoreductase -
CYQ10_RS09110 1805167..1806096 + 930 WP_000803366.1 LysR family transcriptional regulator -
CYQ10_RS09115 1806311..1806373 + 63 WP_010723108.1 membrane protein YoeI -
CYQ10_RS09120 1806363..1807721 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CYQ10_RS09125 1807900..1808958 + 1059 WP_000492346.1 transport protein YeeE -
CYQ10_RS09130 1808972..1809199 + 228 WP_000985272.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T97339 WP_000767829.1 NZ_CP027118:1803960-1804214 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T97339 NZ_CP027118:1803960-1804214 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT97339 WP_001259255.1 NZ_CP027118:1803712-1803963 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT97339 NZ_CP027118:1803712-1803963 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References