Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1564108..1564778 Replicon chromosome
Accession NZ_CP027107
Organism Cronobacter sakazakii strain CS-931

Toxin (Protein)


Gene name higB Uniprot ID A0A7V7RIN4
Locus tag CsakCS931_RS07420 Protein ID WP_007893996.1
Coordinates 1564476..1564778 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A7V7RH32
Locus tag CsakCS931_RS07415 Protein ID WP_007894000.1
Coordinates 1564108..1564449 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CsakCS931_RS07390 1559459..1559995 - 537 WP_004386563.1 YniB family protein -
CsakCS931_RS07395 1560154..1560822 + 669 WP_038859934.1 hexitol phosphatase HxpB -
CsakCS931_RS07400 1561010..1561765 + 756 WP_029038972.1 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD -
CsakCS931_RS07405 1561879..1562469 + 591 WP_007696709.1 metal-dependent hydrolase -
CsakCS931_RS07410 1562605..1563996 + 1392 WP_007696707.1 L-cystine transporter -
CsakCS931_RS07415 1564108..1564449 - 342 WP_007894000.1 HigA family addiction module antidote protein Antitoxin
CsakCS931_RS07420 1564476..1564778 - 303 WP_007893996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CsakCS931_RS07425 1564941..1565171 - 231 WP_014729083.1 cell division activator CedA -
CsakCS931_RS07430 1565383..1567638 + 2256 WP_007893990.1 catalase HPII -
CsakCS931_RS07435 1567757..1568098 - 342 WP_004387341.1 osmotically-inducible lipoprotein OsmE -
CsakCS931_RS07440 1568330..1569157 + 828 WP_004387342.1 ammonia-dependent NAD(+) synthetase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(7-96)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11687.29 Da        Isoelectric Point: 9.6980

>T97307 WP_007893996.1 NZ_CP027107:c1564778-1564476 [Cronobacter sakazakii]
MQKHIGSFRDAWLAAFFVYSTPHRNIPAEIHNTLARKLDIINAATSYLDLRSPPGNRFEALSGKLQGYSSIRVNHHYRLI
FRWVEGKAEDLYLDPHDYRM

Download         Length: 303 bp

>T97307 NZ_CP027107:c1564778-1564476 [Cronobacter sakazakii]
ATGCAGAAGCATATCGGCAGTTTTCGTGACGCCTGGCTGGCGGCTTTTTTCGTGTATTCGACGCCACACCGCAACATTCC
GGCAGAGATTCATAACACACTGGCGCGTAAGCTCGACATCATCAACGCAGCAACCTCATACCTCGATCTCCGCTCGCCGC
CTGGCAACCGGTTTGAGGCGCTGAGCGGTAAATTGCAGGGGTATTCATCGATTCGGGTCAACCATCACTACCGGCTGATT
TTCAGGTGGGTCGAAGGCAAAGCGGAAGACCTGTATTTAGATCCCCATGATTACCGAATGTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12978.91 Da        Isoelectric Point: 7.0171

>AT97307 WP_007894000.1 NZ_CP027107:c1564449-1564108 [Cronobacter sakazakii]
MKQATRKPTTPGEILAYEYLEPLGLRINEVAEMLRVHRNTVSALVNNNRKLTTDMAFRLAKAFGTSVDFWLNLQAAVDLW
EVENDMRIQEEFSRILPAAEFIARRDAQKKDVA

Download         Length: 342 bp

>AT97307 NZ_CP027107:c1564449-1564108 [Cronobacter sakazakii]
ATGAAACAGGCAACCAGAAAGCCCACCACGCCCGGCGAGATCCTCGCTTATGAATACCTGGAACCGCTCGGTCTCAGAAT
TAACGAAGTGGCCGAGATGCTCCGCGTCCATCGCAATACGGTAAGCGCACTGGTAAACAATAACCGCAAGCTCACTACCG
ATATGGCGTTTCGCCTGGCGAAAGCATTCGGCACCTCTGTCGACTTCTGGCTTAACCTGCAGGCGGCTGTCGACCTGTGG
GAAGTGGAAAATGATATGCGCATTCAGGAAGAGTTCAGCCGTATCCTGCCCGCCGCCGAGTTTATCGCCCGGCGCGACGC
GCAGAAGAAAGATGTCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V7RIN4

Antitoxin

Source ID Structure
AlphaFold DB A0A7V7RH32

References