Detailed information of TA system TA097297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 628500..629145
• Replicon: chromosome
• Accession: NZ_CP027107
• Organism: Cronobacter sakazakii strain CS-931

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7V7RET2
• Locus tag: CsakCS931_RS02955
• Protein ID: WP_029039286.1
• Coordinates: 628500..628862 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CsakCS931_RS02960
• Protein ID: WP_175299748.1
• Coordinates: 628849..629145 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CsakCS931_RS02945	623744..627022	-	3279	WP_175299747.1	MdtB/MuxB family multidrug efflux RND transporter permease subunit	-
CsakCS931_RS02950	627022..628269	-	1248	WP_004388207.1	MdtA/MuxA family multidrug efflux RND transporter periplasmic adaptor subunit	-
CsakCS931_RS02955	628500..628862	+	363	WP_029039286.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CsakCS931_RS02960	628849..629145	+	297	WP_175299748.1	helix-turn-helix transcriptional regulator	Antitoxin
CsakCS931_RS02965	629155..630507	-	1353	WP_007902966.1	molecular chaperone	-
CsakCS931_RS02970	630643..631491	+	849	WP_007902962.1	DNA-3-methyladenine glycosylase 2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 14211.10 Da
• Isoelectric Point: 8.0154

>T97297 WP_029039286.1 NZ_CP027107:628500-628862 [Cronobacter sakazakii]
MAWTVIFTDRFNDWYQQQPEGLQDRIAALLWNLRYSGPLVGRPLVDTVKGSRYPNLKELRVQYGGEPWRIFFAFDAERQA
VVLCAGNKRSQKHFYDALIRQAEEEFFLHVQAMERRNENS

Nucleotide

• Length: 363 bp

>T97297 NZ_CP027107:628500-628862 [Cronobacter sakazakii]
ATGGCATGGACGGTGATTTTTACCGATCGTTTTAACGACTGGTATCAGCAGCAGCCCGAAGGATTACAGGATCGTATCGC
CGCGCTCTTATGGAACTTACGTTATTCCGGCCCGCTTGTCGGACGCCCGCTGGTCGATACGGTGAAAGGTTCGCGTTATC
CCAACCTGAAAGAGCTACGTGTGCAATATGGCGGTGAGCCCTGGCGCATTTTTTTTGCGTTTGACGCTGAACGTCAGGCC
GTTGTGCTCTGCGCAGGCAATAAACGAAGCCAGAAACATTTTTATGACGCCTTAATCAGGCAGGCTGAAGAAGAATTCTT
CCTGCATGTGCAAGCGATGGAGAGACGAAATGAAAACTCTTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10780.69 Da
• Isoelectric Point: 5.1548

>AT97297 WP_175299748.1 NZ_CP027107:628849-629145 [Cronobacter sakazakii]
MKTLDDVMAGFSPERQAEILRMADEIALEHGLPLIREARAFSQQQLADIMGVTQPAIAAIEQRGKEIKLLTLKRYVEALG
GKLSLLIELPEGSKVIPV

Nucleotide

• Length: 297 bp

>AT97297 NZ_CP027107:628849-629145 [Cronobacter sakazakii]
ATGAAAACTCTTGATGACGTAATGGCGGGCTTTTCCCCGGAACGGCAGGCAGAAATCCTGCGCATGGCAGACGAAATCGC
GCTGGAGCACGGCCTGCCGCTCATTCGCGAAGCGCGGGCGTTTTCGCAGCAGCAACTGGCGGATATAATGGGCGTGACGC
AGCCCGCTATCGCCGCCATTGAGCAGCGCGGGAAGGAGATCAAACTGCTGACGCTCAAGCGCTACGTTGAAGCGCTGGGC
GGCAAGCTCTCTTTACTGATAGAGCTGCCCGAAGGCAGCAAAGTGATCCCCGTCTGA

Structures

Toxin

• AlphaFold DB: A0A7V7RET2