Detailed information of TA system TA097296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 575080..575727
• Replicon: chromosome
• Accession: NZ_CP027107
• Organism: Cronobacter sakazakii strain CS-931

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CsakCS931_RS02715
• Protein ID: WP_097573525.1
• Coordinates: 575080..575427 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V5TYW5
• Locus tag: CsakCS931_RS02720
• Protein ID: WP_007776551.1
• Coordinates: 575428..575727 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CsakCS931_RS02690	570311..571321	+	1011	WP_004387466.1	galactose/methyl galactoside ABC transporter permease MglC	-
CsakCS931_RS02695	571882..572595	-	714	WP_004387784.1	outer membrane permeability protein SanA	-
CsakCS931_RS02700	572720..573604	-	885	WP_029039355.1	cytidine deaminase	-
CsakCS931_RS02705	573734..574429	-	696	WP_007899537.1	CidB/LrgB family autolysis modulator	-
CsakCS931_RS02710	574426..574827	-	402	WP_007869367.1	CidA/LrgA family protein	-
CsakCS931_RS02715	575080..575427	+	348	WP_097573525.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CsakCS931_RS02720	575428..575727	+	300	WP_007776551.1	XRE family transcriptional regulator	Antitoxin
CsakCS931_RS02725	575800..576732	+	933	WP_097573527.1	tRNA dihydrouridine(16) synthase DusC	-
CsakCS931_RS02730	576735..577658	-	924	WP_054624201.1	LysR family transcriptional regulator	-
CsakCS931_RS02735	577731..578690	+	960	WP_007899546.1	MBL fold metallo-hydrolase	-
CsakCS931_RS02740	578727..578810	-	84	WP_000691708.1	protein YohP	-
CsakCS931_RS02745	578983..579756	+	774	WP_007899548.1	SDR family oxidoreductase	-
CsakCS931_RS02750	579753..580319	-	567	WP_007899550.1	DedA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-83)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13487.74 Da
• Isoelectric Point: 10.0481

>T97296 WP_097573525.1 NZ_CP027107:575080-575427 [Cronobacter sakazakii]
MWTIYLTHEFECWLARQPQPLQEDVLAALGLLKIKGPHLGRPYADTLKGSRHAGMKELRVQHAGRPIRAFYAFDPHRYAI
VLCAAQKKGDEKRFYRVMLRVADKLFSHYLNHWEV

Nucleotide

• Length: 348 bp

>T97296 NZ_CP027107:575080-575427 [Cronobacter sakazakii]
ATGTGGACTATTTACCTTACTCACGAGTTTGAATGCTGGCTCGCGAGACAGCCGCAGCCGTTGCAGGAAGATGTGCTGGC
CGCGCTGGGGCTTTTAAAAATCAAAGGTCCGCACCTCGGGCGTCCGTATGCCGATACGCTCAAAGGCTCGCGTCACGCGG
GTATGAAGGAATTACGGGTTCAGCACGCCGGGCGGCCCATACGCGCCTTTTACGCTTTTGATCCGCACCGCTATGCGATT
GTGCTGTGCGCGGCACAGAAAAAGGGCGATGAAAAGCGGTTTTACCGGGTCATGCTGCGCGTCGCTGACAAACTGTTTAG
CCACTATCTGAACCACTGGGAGGTATAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11035.67 Da
• Isoelectric Point: 5.1667

>AT97296 WP_007776551.1 NZ_CP027107:575428-575727 [Cronobacter sakazakii]
MKTFDEVLAAQTPEARARIEEKTAQLLLETHLYALREALSVSQTEMARQMGIAQPSVAAIEQRGNEMKISTLKRYVEALG
GTVRLDVELPDGQRFGFTL

Nucleotide

• Length: 300 bp

>AT97296 NZ_CP027107:575428-575727 [Cronobacter sakazakii]
ATGAAGACGTTTGATGAAGTGCTGGCGGCGCAGACCCCGGAGGCCCGCGCACGCATCGAAGAGAAAACGGCGCAGCTGCT
GCTGGAAACCCACCTGTATGCGCTGCGCGAGGCGCTCAGCGTGTCGCAAACCGAGATGGCGCGCCAGATGGGTATCGCCC
AGCCGTCAGTCGCGGCTATCGAACAGCGCGGCAACGAGATGAAAATCTCAACGCTTAAGCGCTATGTCGAAGCCCTGGGC
GGCACGGTGCGGCTGGATGTCGAGCTGCCGGACGGCCAGCGTTTCGGTTTTACGCTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2S9UA72