Detailed information of TA system TA097292

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 103641..103895
• Replicon: plasmid pRM14721
• Accession: NZ_CP027106
• Organism: Escherichia coli strain RM14721

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: C5097_RS24945
• Protein ID: WP_001362723.1
• Coordinates: 103641..103847 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	103839..103895 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5097_RS24915	99662..100875	+	1214	WP_139371347.1	IS3 family transposase	-
C5097_RS24925	101941..102798	-	858	WP_158701383.1	incFII family plasmid replication initiator RepA	-
C5097_RS24930	102791..102865	-	75	WP_001375168.1	RepA leader peptide Tap	-
C5097_RS24940	103100..103357	-	258	WP_000083826.1	replication regulatory protein RepA	-
C5097_RS24945	103641..103847	-	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	103839..103895	+	57	NuclAT_1	-	Antitoxin
-	103839..103895	+	57	NuclAT_1	-	Antitoxin
-	103839..103895	+	57	NuclAT_1	-	Antitoxin
-	103839..103895	+	57	NuclAT_1	-	Antitoxin
C5097_RS24955	104070..104354	-	285	WP_106668553.1	hypothetical protein	-
C5097_RS24960	104544..105275	-	732	WP_106668554.1	type-F conjugative transfer system pilin acetylase TraX	-
C5097_RS24965	105272..105838	-	567	WP_000469208.1	DUF2726 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cofA	1..106432	106432

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7792.29 Da
• Isoelectric Point: 8.8807

>T97292 WP_001362723.1 NZ_CP027106:c103847-103641 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T97292 NZ_CP027106:c103847-103641 [Escherichia coli]
ATGAAGTACTTGAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGTGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAATAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT97292 NZ_CP027106:103839-103895 [Escherichia coli]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin