Detailed information of TA system TA097265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 4496059..4497377
• Replicon: chromosome
• Accession: NZ_CP027103
• Organism: Escherichia coli strain RM14723

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: C5099_RS23400
• Protein ID: WP_021560080.1
• Coordinates: 4496059..4497066 (-)
• Length: 336 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A2A7M399
• Locus tag: C5099_RS23405
• Protein ID: WP_001490601.1
• Coordinates: 4497066..4497377 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5099_RS23365	4492604..4492711	-	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
C5099_RS23375	4492783..4493817	+	1035	Protein_4281	transposase	-
C5099_RS23380	4494188..4494295	-	108	WP_000170747.1	type I toxin-antitoxin system toxin Ldr family protein	-
C5099_RS23395	4494771..4496042	+	1272	WP_001312176.1	amino acid permease	-
C5099_RS23400	4496059..4497066	-	1008	WP_021560080.1	HipA domain-containing protein	Toxin
C5099_RS23405	4497066..4497377	-	312	WP_001490601.1	HipA N-terminal domain-containing protein	Antitoxin
C5099_RS23410	4497362..4497685	-	324	WP_021560081.1	helix-turn-helix transcriptional regulator	-
C5099_RS23415	4497910..4498923	-	1014	WP_001357946.1	dipeptide ABC transporter ATP-binding subunit DppF	-
C5099_RS23420	4498920..4499903	-	984	WP_001196477.1	dipeptide ABC transporter ATP-binding protein	-
C5099_RS23425	4499914..4500816	-	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
C5099_RS23430	4500826..4501845	-	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (56-302)

Antitoxin

• HipA, N-terminal subdomain 1 (6-98)

Sequences

Toxin

Protein

• Length: 336 a.a.
• Molecular weight: 38395.65 Da
• Isoelectric Point: 5.4135

>T97265 WP_021560080.1 NZ_CP027103:c4497066-4496059 [Escherichia coli]
MANCRILLTPLNERDEQRGYSTQGLKRLSGTAKLTPRLGFTRTQFVQELPRQQKGMSISGYQPKLQLVLDEGEFRVVDHQ
GNFILKPSPADFPGLAENEHATMTLMSRLGFDVPVHGLLSFAPQSEEELEYAFVIRRYDRDYKGLPVHQEQLDGAMQITD
KYGKTGNDNEQYVSYETLARFLVAHVNDNIAFKIDLFRRIVYAWLLGNNDMHLRNFGLVYSDGLTPTLAPVYDFVSVAPY
PEYFYSNYLALPLLTREEGGRELAPGFHSDYGEYIGQDFLLLGESMGLAPRLLEKLFQDIRKENAIVMETYEQSFMTQDH
IQAVLQCYRHRLGLL

Nucleotide

• Length: 1008 bp

>T97265 NZ_CP027103:c4497066-4496059 [Escherichia coli]
ATGGCGAATTGTCGTATTCTGTTAACGCCGTTGAATGAACGTGACGAACAGCGCGGATATTCGACACAGGGGTTGAAGCG
ACTGAGCGGCACTGCAAAATTGACCCCCAGGCTGGGGTTTACGCGAACTCAGTTTGTGCAGGAACTTCCGCGTCAACAAA
AAGGGATGAGTATTTCCGGTTACCAGCCCAAATTGCAATTAGTGCTGGATGAAGGAGAGTTCCGCGTCGTAGATCATCAG
GGAAATTTTATCCTTAAGCCCTCTCCAGCCGACTTTCCCGGCTTGGCCGAAAATGAACACGCAACAATGACATTGATGTC
TCGTTTGGGATTTGACGTACCTGTGCATGGCCTGTTGAGTTTTGCACCACAATCAGAGGAAGAGCTTGAATACGCATTTG
TTATCCGGCGCTATGACCGGGATTACAAGGGGCTGCCAGTGCATCAGGAACAGCTTGATGGCGCGATGCAGATTACAGAT
AAATATGGCAAAACGGGGAATGATAACGAACAATATGTCAGCTATGAAACGCTGGCCCGATTTTTAGTTGCCCATGTTAA
CGACAACATCGCATTTAAAATCGATCTCTTTCGTCGCATTGTTTACGCCTGGTTGCTGGGTAATAACGACATGCATTTAC
GAAACTTTGGTTTAGTGTACTCCGATGGTTTGACGCCAACGTTAGCGCCGGTGTATGACTTTGTTTCTGTCGCTCCCTAT
CCGGAATATTTTTACTCAAACTATCTGGCGCTGCCGTTATTGACGCGAGAAGAAGGCGGTCGGGAACTGGCTCCTGGATT
TCACAGTGATTATGGCGAGTATATCGGGCAGGACTTTTTGCTGTTGGGTGAATCAATGGGGTTAGCGCCCAGATTGCTGG
AAAAACTCTTTCAGGATATACGTAAAGAAAATGCCATTGTTATGGAAACGTATGAGCAGTCCTTTATGACTCAGGATCAT
ATCCAGGCTGTGCTGCAATGCTATCGACATCGTCTGGGGCTGCTGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11822.40 Da
• Isoelectric Point: 6.0609

>AT97265 WP_001490601.1 NZ_CP027103:c4497377-4497066 [Escherichia coli]
MHRRVKVLLYGQVVGELSQNDSGFLFQYAQDYHGPAISISLPVAQRQFPSETLHPYFASLAPEGWLRQRYSQIQHRDEND
LLGMLIDNGKNLLGAIQILPWEE

Nucleotide

• Length: 312 bp

>AT97265 NZ_CP027103:c4497377-4497066 [Escherichia coli]
ATGCATCGACGAGTGAAAGTGTTGCTCTATGGTCAGGTAGTGGGTGAACTCTCTCAGAACGACAGTGGTTTTTTGTTCCA
GTACGCCCAGGATTACCACGGACCGGCAATCTCAATCAGCCTGCCCGTCGCACAGCGTCAGTTTCCCAGTGAGACGTTGC
ATCCTTATTTCGCCTCACTGGCGCCAGAAGGATGGCTGCGTCAACGCTACAGCCAGATACAGCATCGTGACGAAAACGAT
CTGCTGGGAATGCTTATTGATAACGGCAAGAATCTGCTGGGGGCAATCCAGATTTTACCTTGGGAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2A7M399