Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3611261..3611988 Replicon chromosome
Accession NZ_CP027103
Organism Escherichia coli strain RM14723

Toxin (Protein)


Gene name higB Uniprot ID Q3YYE6
Locus tag C5099_RS18865 Protein ID WP_000547563.1
Coordinates 3611677..3611988 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A3R1A2R4
Locus tag C5099_RS18860 Protein ID WP_021559999.1
Coordinates 3611261..3611680 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5099_RS18840 3607424..3609676 - 2253 WP_021559998.1 carbamoyltransferase HypF -
C5099_RS18845 3609829..3610356 - 528 WP_001078777.1 electron transport protein HydN -
C5099_RS18855 3610741..3611169 + 429 WP_000536064.1 hypothetical protein -
C5099_RS18860 3611261..3611680 - 420 WP_021559999.1 helix-turn-helix domain-containing protein Antitoxin
C5099_RS18865 3611677..3611988 - 312 WP_000547563.1 type II toxin-antitoxin system HigB family toxin Toxin
C5099_RS18870 3612151..3612906 - 756 Protein_3451 hypothetical protein -
C5099_RS18875 3612949..3613419 - 471 WP_001357572.1 hydrogenase maturation peptidase HycI -
C5099_RS18880 3613412..3613822 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
C5099_RS18885 3613819..3614586 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
C5099_RS18890 3614586..3615128 - 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
C5099_RS18895 3615138..3616847 - 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12398.11 Da        Isoelectric Point: 9.7105

>T97260 WP_000547563.1 NZ_CP027103:c3611988-3611677 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T97260 NZ_CP027103:c3611988-3611677 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15446.45 Da        Isoelectric Point: 4.4596

>AT97260 WP_021559999.1 NZ_CP027103:c3611680-3611261 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GAALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT97260 NZ_CP027103:c3611680-3611261 [Escherichia coli]
ATGACTGCTAATGCTGTACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGCGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QA22

Antitoxin

Source ID Structure
AlphaFold DB A0A3R1A2R4

References