97248

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1252988..1253667 Replicon chromosome
Accession NZ_CP027103
Organism Escherichia coli strain RM14723

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag C5099_RS06325 Protein ID WP_000057523.1
Coordinates 1252988..1253290 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag C5099_RS06330 Protein ID WP_000806442.1
Coordinates 1253326..1253667 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5099_RS06295 1248251..1249471 - 1221 WP_001251634.1 fosmidomycin MFS transporter -
C5099_RS06305 1249689..1251341 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
C5099_RS06310 1251378..1251857 - 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
C5099_RS06320 1252061..1252855 - 795 WP_000365161.1 TraB/GumN family protein -
C5099_RS06325 1252988..1253290 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C5099_RS06330 1253326..1253667 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
C5099_RS06335 1253725..1256229 - 2505 WP_000084001.1 copper-exporting P-type ATPase CopA -
C5099_RS06340 1256492..1257424 + 933 WP_000883035.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T97248 WP_000057523.1 NZ_CP027103:1252988-1253290 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T97248 NZ_CP027103:1252988-1253290 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT97248 WP_000806442.1 NZ_CP027103:1253326-1253667 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT97248 NZ_CP027103:1253326-1253667 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References