Detailed information of TA system TA097241

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 718663..719072
• Replicon: chromosome
• Accession: NZ_CP027103
• Organism: Escherichia coli strain RM14723

Toxin (Protein)

• Gene name: symE
• Uniprot ID: A0A3L4RJH9
• Locus tag: C5099_RS03640
• Protein ID: WP_001357525.1
• Coordinates: 718663..719001 (-)
• Length: 113 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	718996..719072 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5099_RS03615	714322..715473	-	1152	WP_001357520.1	2-hydroxyacyl-CoA dehydratase subunit D	-
C5099_RS03620	715589..716868	-	1280	Protein_644	DUF445 domain-containing protein	-
C5099_RS03625	717053..717973	+	921	WP_001357522.1	Rpn family recombination-promoting nuclease/putative transposase	-
C5099_RS03630	717956..718279	-	324	WP_001357523.1	winged helix-turn-helix domain-containing protein	-
C5099_RS03635	718457..718621	+	165	WP_001357524.1	DUF1127 domain-containing protein	-
C5099_RS03640	718663..719001	-	339	WP_001357525.1	endoribonuclease SymE	Toxin
-	718996..719072	+	77	NuclAT_5	-	Antitoxin
-	718996..719072	+	77	NuclAT_5	-	Antitoxin
-	718996..719072	+	77	NuclAT_5	-	Antitoxin
-	718996..719072	+	77	NuclAT_5	-	Antitoxin
-	718996..719072	+	77	NuclAT_6	-	Antitoxin
-	718996..719072	+	77	NuclAT_6	-	Antitoxin
-	718996..719072	+	77	NuclAT_6	-	Antitoxin
-	718996..719072	+	77	NuclAT_6	-	Antitoxin
C5099_RS03645	719216..719884	-	669	Protein_649	DUF3387 domain-containing protein	-
C5099_RS03650	719878..720051	-	174	Protein_650	SAM-dependent DNA methyltransferase	-
C5099_RS03655	720096..721262	-	1167	WP_000800830.1	restriction endonuclease	-
C5099_RS03660	721358..721567	+	210	WP_001534843.1	BrnT family toxin	-
C5099_RS03665	721564..721866	+	303	WP_000063155.1	BrnA antitoxin family protein	-
C5099_RS03670	721900..722856	-	957	WP_001314406.1	GTPase	-
C5099_RS03675	722867..723070	-	204	WP_000467859.1	YbdD/YjiX family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12314.03 Da
• Isoelectric Point: 7.8234

>T97241 WP_001357525.1 NZ_CP027103:c719001-718663 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFTTGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQRQVQDFIGVISSKTPR

Nucleotide

• Length: 339 bp

>T97241 NZ_CP027103:c719001-718663 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGCCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTACCA
CCGGCACGGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAAGAGAGCGAA
CTGATGCAGTCACTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGATTTTATTGGGGTGATTAG
TAGCAAAACGCCACGCTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT97241 NZ_CP027103:718996-719072 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A3L4RJH9

Antitoxin