Detailed information of TA system TA097239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 167866..168468
• Replicon: chromosome
• Accession: NZ_CP027103
• Organism: Escherichia coli strain RM14723

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: C5099_RS00830
• Protein ID: WP_000897302.1
• Coordinates: 167866..168177 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C5099_RS00835
• Protein ID: WP_000356397.1
• Coordinates: 168178..168468 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5099_RS00805	163780..164379	+	600	WP_001298594.1	glucose-1-phosphatase	-
C5099_RS00810	164373..165245	+	873	WP_000920762.1	virulence factor BrkB family protein	-
C5099_RS00815	165242..165679	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C5099_RS00820	165724..166665	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C5099_RS00825	166729..167637	-	909	WP_001357065.1	alpha/beta hydrolase	-
C5099_RS00830	167866..168177	+	312	WP_000897302.1	hypothetical protein	Toxin
C5099_RS00835	168178..168468	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C5099_RS00845	168827..169105	+	279	WP_001296612.1	hypothetical protein	-
C5099_RS00855	169501..169719	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
C5099_RS00860	169943..170872	-	930	WP_000027710.1	formate dehydrogenase accessory protein FdhE	-
C5099_RS00865	170869..171504	-	636	WP_001357064.1	formate dehydrogenase cytochrome b556 subunit	-
C5099_RS00870	171501..172403	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T97239 WP_000897302.1 NZ_CP027103:167866-168177 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T97239 NZ_CP027103:167866-168177 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT97239 WP_000356397.1 NZ_CP027103:168178-168468 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT97239 NZ_CP027103:168178-168468 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416