Detailed information of TA system TA097217

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 118978..119231
• Replicon: plasmid pKPC2_095084
• Accession: NZ_CP027067
• Organism: Klebsiella pneumoniae strain WCHKP8F4

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: B7D38_RS01105
• Protein ID: WP_001312851.1
• Coordinates: 119082..119231 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	118978..119037 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D38_RS01060	114638..114703	-	66	Protein_187	hypothetical protein	-
B7D38_RS01065	114756..115460	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
B7D38_RS01070	115524..115685	+	162	Protein_189	DNA helicase	-
B7D38_RS01075	115705..116451	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
B7D38_RS01080	116506..117066	+	561	WP_000139328.1	fertility inhibition protein FinO	-
B7D38_RS01085	117198..117398	+	201	WP_015059022.1	hypothetical protein	-
B7D38_RS01090	117784..118383	+	600	WP_032083981.1	hypothetical protein	-
B7D38_RS01095	118547..118777	+	231	WP_001736714.1	hypothetical protein	-
B7D38_RS01100	118800..119027	-	228	Protein_195	hypothetical protein	-
-	118978..119037	-	60	NuclAT_1	-	Antitoxin
-	118978..119037	-	60	NuclAT_1	-	Antitoxin
-	118978..119037	-	60	NuclAT_1	-	Antitoxin
-	118978..119037	-	60	NuclAT_1	-	Antitoxin
B7D38_RS01105	119082..119231	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
B7D38_RS01110	119515..119763	+	249	WP_000083837.1	replication regulatory protein RepA	-
B7D38_RS01115	119764..119973	-	210	WP_064765370.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2	-	1..120074	120074

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T97217 WP_001312851.1 NZ_CP027067:119082-119231 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T97217 NZ_CP027067:119082-119231 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT97217 NZ_CP027067:c119037-118978 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin