Detailed information of TA system TA097212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4088547..4089144
• Replicon: chromosome
• Accession: NZ_CP027064
• Organism: Klebsiella variicola strain WCHKV030666

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: CLQ71_RS21565
• Protein ID: WP_012542526.1
• Coordinates: 4088827..4089144 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CLQ71_RS21560
• Protein ID: WP_012542525.1
• Coordinates: 4088547..4088834 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ71_RS21530	4084457..4084705	+	249	WP_008805539.1	DUF1158 domain-containing protein	-
CLQ71_RS21535	4084722..4085063	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
CLQ71_RS21540	4085094..4086209	-	1116	WP_162493283.1	MBL fold metallo-hydrolase	-
CLQ71_RS21545	4086389..4086970	+	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
CLQ71_RS21550	4086970..4087338	+	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
CLQ71_RS21555	4087458..4088111	+	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
CLQ71_RS21560	4088547..4088834	-	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
CLQ71_RS21565	4088827..4089144	-	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CLQ71_RS21570	4089329..4090372	-	1044	WP_016160437.1	DUF2157 domain-containing protein	-
CLQ71_RS21575	4090902..4091768	-	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
CLQ71_RS21580	4091877..4093304	+	1428	WP_101837261.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T97212 WP_012542526.1 NZ_CP027064:c4089144-4088827 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T97212 NZ_CP027064:c4089144-4088827 [Klebsiella variicola]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT97212 WP_012542525.1 NZ_CP027064:c4088834-4088547 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT97212 NZ_CP027064:c4088834-4088547 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2