Detailed information of TA system TA097127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3749..4406
• Replicon: plasmid IncAC2
• Accession: NZ_CP027038
• Organism: Klebsiella pneumoniae strain 16_GR_13

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: A6D82_RS28350
• Protein ID: WP_000270043.1
• Coordinates: 3749..4099 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A6D82_RS28355
• Protein ID: WP_000124640.1
• Coordinates: 4104..4406 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6D82_RS28310	223..720	-	498	WP_000062185.1	hypothetical protein	-
A6D82_RS28315	723..1211	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
A6D82_RS28320	1308..1643	+	336	WP_000683476.1	hypothetical protein	-
A6D82_RS28325	1658..2128	-	471	WP_001281821.1	hypothetical protein	-
A6D82_RS28330	2121..2492	-	372	WP_000516916.1	hypothetical protein	-
A6D82_RS28335	2503..2697	-	195	WP_000343597.1	hypothetical protein	-
A6D82_RS28345	3038..3586	-	549	WP_001061574.1	transcriptional regulator	-
A6D82_RS28350	3749..4099	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A6D82_RS28355	4104..4406	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
A6D82_RS28360	4433..4726	-	294	WP_001239997.1	chromosome segregation protein ParM	-
A6D82_RS28365	4814..5086	-	273	WP_001043047.1	HU family DNA-binding protein	-
A6D82_RS28370	5144..5671	-	528	WP_001236377.1	thermonuclease family protein	-
A6D82_RS28380	5902..6759	-	858	WP_001167032.1	hypothetical protein	-
A6D82_RS28385	6746..6976	-	231	WP_000972663.1	hypothetical protein	-
A6D82_RS28390	6976..7494	-	519	WP_000210756.1	nitrite reductase	-
A6D82_RS28395	7491..7937	-	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
A6D82_RS28400	7937..8296	-	360	WP_000422768.1	hypothetical protein	-
A6D82_RS28405	8353..8781	-	429	WP_000591074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaTEM-1B / rmtB / tet(G) / floR / qacE / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / ant(2'')-Ia / blaVEB-1	-	1..155161	155161

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T97127 WP_000270043.1 NZ_CP027038:3749-4099 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T97127 NZ_CP027038:3749-4099 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT97127 WP_000124640.1 NZ_CP027038:4104-4406 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT97127 NZ_CP027038:4104-4406 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9