Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3518254..3518924 Replicon chromosome
Accession NZ_CP027035
Organism Mycobacterium tuberculosis variant bovis strain 2002/0476

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag C1D11_RS17305 Protein ID WP_003899954.1
Coordinates 3518254..3518598 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag C1D11_RS17310 Protein ID WP_003899955.1
Coordinates 3518595..3518924 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1D11_RS17270 3513327..3514187 + 861 WP_003416628.1 alpha/beta hydrolase -
C1D11_RS17275 3514282..3514677 + 396 WP_071854247.1 nitroreductase family deazaflavin-dependent oxidoreductase -
C1D11_RS17285 3514917..3515210 + 294 WP_003416635.1 hypothetical protein -
C1D11_RS17290 3515498..3516787 + 1290 WP_003416640.1 ATP-binding protein -
C1D11_RS17295 3517134..3517568 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
C1D11_RS17300 3517571..3518023 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
C1D11_RS17305 3518254..3518598 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C1D11_RS17310 3518595..3518924 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
C1D11_RS17315 3519462..3519809 + 348 WP_003899956.1 DUF2384 domain-containing protein -
C1D11_RS17320 3519806..3520426 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
C1D11_RS17325 3520586..3521851 - 1266 WP_003909837.1 hypothetical protein -
C1D11_RS17330 3522019..3522228 + 210 WP_003416778.1 hypothetical protein -
C1D11_RS17340 3522475..3523509 - 1035 WP_044292644.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T97104 WP_003899954.1 NZ_CP027035:3518254-3518598 [Mycobacterium tuberculosis variant bovis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T97104 NZ_CP027035:3518254-3518598 [Mycobacterium tuberculosis variant bovis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT97104 WP_003899955.1 NZ_CP027035:3518595-3518924 [Mycobacterium tuberculosis variant bovis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT97104 NZ_CP027035:3518595-3518924 [Mycobacterium tuberculosis variant bovis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References