Detailed information of TA system TA097104

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3518254..3518924
• Replicon: chromosome
• Accession: NZ_CP027035
• Organism: Mycobacterium tuberculosis variant bovis strain 2002/0476

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: C1D11_RS17305
• Protein ID: WP_003899954.1
• Coordinates: 3518254..3518598 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: C1D11_RS17310
• Protein ID: WP_003899955.1
• Coordinates: 3518595..3518924 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1D11_RS17270	3513327..3514187	+	861	WP_003416628.1	alpha/beta hydrolase	-
C1D11_RS17275	3514282..3514677	+	396	WP_071854247.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
C1D11_RS17285	3514917..3515210	+	294	WP_003416635.1	hypothetical protein	-
C1D11_RS17290	3515498..3516787	+	1290	WP_003416640.1	ATP-binding protein	-
C1D11_RS17295	3517134..3517568	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
C1D11_RS17300	3517571..3518023	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
C1D11_RS17305	3518254..3518598	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C1D11_RS17310	3518595..3518924	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
C1D11_RS17315	3519462..3519809	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
C1D11_RS17320	3519806..3520426	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
C1D11_RS17325	3520586..3521851	-	1266	WP_003909837.1	hypothetical protein	-
C1D11_RS17330	3522019..3522228	+	210	WP_003416778.1	hypothetical protein	-
C1D11_RS17340	3522475..3523509	-	1035	WP_044292644.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T97104 WP_003899954.1 NZ_CP027035:3518254-3518598 [Mycobacterium tuberculosis variant bovis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T97104 NZ_CP027035:3518254-3518598 [Mycobacterium tuberculosis variant bovis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT97104 WP_003899955.1 NZ_CP027035:3518595-3518924 [Mycobacterium tuberculosis variant bovis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT97104 NZ_CP027035:3518595-3518924 [Mycobacterium tuberculosis variant bovis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6